云南民族大学学报（自然科学版）2025，Vol.34Issue(5)：514-521,8.DOI:10.3969/j.issn.1672-8513.2025.05.002
锅铲叶总黄酮提取工艺及体外抗氧化活性研究
Study on extraction technology and antioxidant activity in vitro of total flavonoids from Passiflora wilsonii Hemsl foliage
摘要
Abstract
Taking Passiflora wilsonii Hemsl foliage as raw material and based on single factor experiment,the effects of ethanol volume fraction,liquid-solid ratio,extraction time and extraction temperature on the yield of total flavonoids from P.wilsonii Hemsl foliage were investigated.The antioxidant capacity of total flavonoids from P.wilsonii Hemsl was evaluated by DPPH,ABTS and FRAP,vitamin C and rutin were used as positive controls.The results showed that the yield of total flavonoids was significantly affected by liquid-solid ratio,extraction temperature and extraction time.With 70%ethanol solution as solvent,liquid-solid ratio of 27 mL/g,and extraction at 61℃for 42 min,the average yield of flavonoids was 23.448 5 mg/g.The extracted total flavonoids had good scavenging ability for DPPH,ABTS+and Fe3+-TPTZ,providing an valuable research foundation for further analysis of the chemical composition and activity of P.wilsonii Hemsl foliage.关键词
锅铲叶/总黄酮/提取工艺/响应面法/抗氧化Key words
Passiflora wilsonii Hemsl/total flavonoids/extraction technology/response-surface methodology/antioxidant分类
医药卫生引用本文复制引用
李青霞,周兴杨,杨发建,李红玉,赵兴蕊..锅铲叶总黄酮提取工艺及体外抗氧化活性研究[J].云南民族大学学报（自然科学版）,2025,34(5):514-521,8.基金项目
云南省教育厅科学研究基金(2023J1804) （2023J1804）
2022年市级重点领域自筹经费科技计划(2022zc32). （2022zc32）
