云南民族大学学报（自然科学版）2025，Vol.34Issue(5)：531-537,7.DOI:10.3969/j.issn.1672-8513.2025.05.004
美洲大蠊多肽提取工艺优化及免疫活性验证
Optimization of Periplaneta americana polypeptide extraction process and verification of immune activity for Periplaneta americana
摘要
Abstract
In order to explore the optimum extraction process of Periplaneta americana polypeptide and evaluate its immune activity,three different extraction methods of ultrasonic cell disruption,trypsin digestion and neutral protease digestion were compared by using Periplaneta americana as the raw material.It was found that trypsin digestion had the highest extraction efficiency.The effects of enzyme-to-substrate ratio,solid-liquid ratio and ultrasonic time on the yield of Periplaneta americana polypeptide were further investigated.The effects of Periplaneta americana polypeptide on the proliferation and phagocytic activity of RAW264.7 cells were detected by CCK-8 method and neutral red phagocytosis assay.The results showed that the yield of Periplaneta americana polypeptide was(11.92±0.31)%under the conditions of enzyme-substrate ratio 5 000 U/g,solid-liquid ratio 1∶20 g/mL and ultrasonic time 5 h.The results of CCK-8 and neutral red phagocytosis experiments showed that RAW264.7 cells had better proliferation and phagocytic activity when the mass concentration was 5 to 40 μg/mL.The results of in vivo experiments also showed that Periplaneta americana polypeptide showed good immune activity in mice.关键词
美洲大蠊/多肽/提取工艺/免疫活性Key words
Periplaneta americana/polypeptide/extraction process/immune activity分类
医药卫生引用本文复制引用
郝金旺,胡晶红,王海荣,吴建龙,陈文潇,洪永健,张笑瑜,郗宇腾,徐志玮..美洲大蠊多肽提取工艺优化及免疫活性验证[J].云南民族大学学报（自然科学版）,2025,34(5):531-537,7.基金项目
山东省重点研发计划(2022TZXD0035) （2022TZXD0035）
日照市重点研发计划(2021ZDYF010118). （2021ZDYF010118）
