云南民族大学学报（自然科学版）2025，Vol.34Issue(5)：564-571,8.DOI:10.3969/j.issn.1672-8513.2025.05.009
复方南板蓝根片HPLC指纹图谱及多指标成分分析
HPLC fingerprint and simultaneous determination of multiple components in compound nanbanlangen tablets
摘要
Abstract
High performance liquid chromatography(HPLC)was used to simultaneously determine the mass fraction of cichorigenin,caffeic acid and cichoric acid,to establish the fingerprints of HPLC,and to combine with chemical pattern recognition for the overall evaluation of the quality of the compound nanbanlangen tablets.The HPLC fingerprints of the 11 batches of compound nanbanlangen tablets were established.Among the 13 common peaks,peak 3 was cichorigenin,peak 4 as caffeic acid,and peak 8 as cichoric acid,and the similarity of the 11 batches of compound nanbanlangen tablets was greater than 0.900.The separation of Cichorigenin,caffeic acid and cichoric acid was effective,with a good linear relationship within their respective ranges(r≥0.999 5).The average recovery rates were 97.52%,99.80%,and 100.96%,and the corresponding relative standard deviations(RSD)were 1.15%,0.85%,and 1.32%,respectively.Cluster analysis was performed to classify the 11 batches of compound nanbanlangen tablets into 2 and 4 categories.The method established was simple,reproducible,sensitive and reliable,which can provide a reference basis for the supervision and testing of the compound nanbanlangen tablets.关键词
复方南板蓝根片/高效液相色谱法/指纹图谱/主成分分析/偏最小二乘判别分析Key words
compound nanbanlangen tablets/high performance liquid chromatography/fingerprinting/principal component analysis/partial least squares discriminant analysis分类
医药卫生引用本文复制引用
古炳明,邝智龙,杨敏斌,刘佳,李艳媚,刘晖琳..复方南板蓝根片HPLC指纹图谱及多指标成分分析[J].云南民族大学学报（自然科学版）,2025,34(5):564-571,8.基金项目
广东省药品监督管理局2022年科技创新项目(2022TDB34) （2022TDB34）
广东省药品监督管理局办公室2022年市县药品监管综合改革创新项目(ZG-DS-2022018). （ZG-DS-2022018）
