湖北医药学院学报2025，Vol.44Issue(5)：519-524,6.DOI:10.13819/j.issn.2096-708X.2025.05.001
丁香酚抑制肺炎克雷伯菌毒力因子的表达及其机制研究
Effect and Mechanism of Eugenol on Inhibiting the Virulence of Klebsiella pneumoniae
摘要
Abstract
Objective To investigate the effect and mechanism of eugenol on the growth and virulence of Klebsiella pneumon-iae.Methods K.pneumoniae was inoculated into liquid culture media containing different concentrations(0,125,250 μg/mL)of eugenol.The growth curve and survival rate of the bacteria were measured,and changes in the main virulence fac-tors,including the productions of capsules,pili,and biofilms,were detected.Furthermore,qRT-PCR was performed to determine the mRNA expressions of virulence-related genes.Results After the treatment by eugenol,the growth rate and the survival rate of K.pneumoniae significantly decreased,and these changes were correlated with the dose of eugenol.Ad-ditionally,under the intervention of eugenol,the productions of capsules,pili,and biofilms by K.pneumoniae decreased in a dose-dependent manner.The qRT-PCR indicated that capsule production-related genes(rmpA,magA),biofilm-related gene(bsmA),and pilus synthesis genes(fimA,fimK)were all down-regulated.Moreover,the expression of the global transcription factor(crp)was significantly reduced.Conclusion Eugenol can inhibit the productions of bacterial capsules,pili,and biofilms by acting on CRP.This study provide a theoretical basis and reference for the clinical application of euge-nol as a potential drug to treat K.pneumoniae-induced infectious diseases.关键词
肺炎克雷伯菌/丁香酚/毒力/荚膜/生物膜/菌毛Key words
Klebsiella pneumoniae/Eugenol/Virulence/Capsule/Biofilm/Pili引用本文复制引用
邱雪梅,刘思雨,王滨,黄昵琪,廖硕,唐颖,汪静杰..丁香酚抑制肺炎克雷伯菌毒力因子的表达及其机制研究[J].湖北医药学院学报,2025,44(5):519-524,6.基金项目
国家自然科学基金(82202537) （82202537）
湖北省"十四五"优势学科群项目(2025BMXKQY4) （2025BMXKQY4）
湖北省大学生创新创业项目(X202410929055) （X202410929055）
湖北医药学院研究生科技创新项目(YC2024011) （YC2024011）
