湖北医药学院学报2025，Vol.44Issue(5)：544-549,6.DOI:10.13819/j.issn.2096-708X.2025.05.005
双硫死亡关键调控基因TCGA/GTEx联合队列分析揭示HNSCC预后生物标志物及潜在治疗靶点
Key Regulatory Genes of Disulfidptosis in a TCGA/GTEx Combined Cohort Reveals Prognostic Biomarkers and Potential Therapeutic Targets for Head and Neck Squamous Cell Carcinoma
摘要
Abstract
Objective To systematically analyze the prognostic value and immunoregulatory mechanisms of disulfidptosis-re-lated long non-coding RNAs(DRLs)in head and neck squamous cell carcinoma(HNSCC).Methods An integrated anal-ysis was performed on 511 HNSCC samples from TCGA and 44 normal tissues from GTEx.DRLs were screened using Pear-son correlation analysis(|R|＞0.4,P＜0.001),followed by univariate COX regression(P＜0.05)and LASSO-COX regres-sion(10-fold cross-validation)to construct a prognostic model.Model performance was evaluated by Kaplan-Meier analy-sis,ROC curves(1/3/5-year AUC),and PCA.Immune microenvironment was assessed via ssGSEA,ESTIMATE,and TIDE analysis.RT-PCR was used to validate the expression of DRLs.Results A prognostic model incorporating six DRLs(AC083967.1,AC106820.5,etc.)was successfully established(3-year AUC=0.716).The high-risk group exhibited de-creased immune scores(P＜0.05)and elevated TIDE scores(P＜0.05),indicating a tendency for immune evasion.Func-tional enrichment analysis revealed that DRLs were involved in antigen presentation and NOD-like receptor pathways(FDR＜0.05).Experimental validation confirmed differential expression of all six DRLs in HNSCC cell lines(vs.HOK,P＜0.05).Conclusion DRLs serve as promising prognostic biomarkers for HNSCC and can regulate the immune microenviron-ment,offering novel targets for personalized therapy.关键词
头颈部鳞状细胞癌/双硫死亡/TCGA/GTEx/预后标志物/免疫微环境/生物信息学Key words
Head and neck squamous cell carcinoma/Disulfidptosis/TCGA/GTEx/Prognostic biomarker/Immune mi-croenvironment/Bioinformatics引用本文复制引用
殷姿,祝常青,孟盼盼,李巧琴,李凯..双硫死亡关键调控基因TCGA/GTEx联合队列分析揭示HNSCC预后生物标志物及潜在治疗靶点[J].湖北医药学院学报,2025,44(5):544-549,6.基金项目
湖北省自然科学基金项目(2024AFD102) （2024AFD102）
十堰市科技局项目(24Y015) （24Y015）
