现代畜牧科技Issue(10)：30-33,4.DOI:10.19369/j.cnki.2095-9737.2025.10.008
凡纳滨对虾Crustin基因生物信息学分析
Bioinformatics Analysis and Tissue Expression of Crustin Gene in Litopenaeus vannamei
摘要
Abstract
This study performed a bioinformatics analysis of the Litopenaeus vannamei Crustin gene.The cDNA sequence was obtained from NCBI,and multiple online tools were used to predict its physicochemical properties,structural features,transmembrane regions,signal peptide,subcellular localization,phosphorylation sites,and protein interactions.The results showed that the protein has a molecular weight of approximately 15.31 kDa with an isoelectric point of 8.00,is generally stable,and exhibits slight hydrophobicity.Both secondary and tertiary structures were predominantly random coils.No transmembrane regions were detected,but a potential signal peptide was identified,suggesting it may be a secretory protein.Subcellular localization predicted it to be mainly distributed in the cytoplasm with potential extracellular transport.Phosphorylation analysis revealed multiple sites potentially involved in several signaling pathways.Protein interaction analysis indicated associations with antimicrobial proteins containing WAP domains.These findings provide a theoretical basis for further investigation into the structural characteristics and immune-related functions of Crustin.关键词
凡纳滨对虾/Crustin基因/抗菌肽/生物信息学Key words
Litopenaeus vannamei/Crustin gene/antimicrobial peptide/bioinformatics分类
农业科技引用本文复制引用
陈雨欢,武静,杨森源,张善诚,王思宇,石丰运,张海涛..凡纳滨对虾Crustin基因生物信息学分析[J].现代畜牧科技,2025,(10):30-33,4.基金项目
江苏省高校基础科学(自然科学)研究面上项目(24KJB240003) （自然科学）
2024年江苏省职业院校学生创新创业培育计划项目(GX-2024-0442) （GX-2024-0442）
