针刺研究2025，Vol.50Issue(10)：1143-1151,9.DOI:10.13702/j.1000-0607.20240816
艾灸调控下丘脑cAMP/PKA/CREB信号通路对肥胖小鼠脂肪组织棕色化的影响
Effects of moxibustion on the browning of adipose tissue in obese mice by regulating the hypothalamic cAMP/PKA/CREB signaling pathway
摘要
Abstract
Objective To explore the mechanisms of moxibustion in treating obese mice through its regulatory effects on mitochondrial brown adipose tissue uncoupling protein-1(UCP1),PR domain-containing protein 16(Prdm16),and peroxisome proliferator-activated receptor gamma coactivator 1-alpha(PGC-1α)in abdominal adipose tissue,and the cyclic adenosine monophosphate(cAMP)/protein kinase A(PKA)/cAMP response element-binding protein(CREB)signaling pathway in the hypothalamus of obese mice.Methods C57BL/6J mice were randomly divided into the control group,model group,moxibustion group,and sham control group,with 10 mice in each group.An obese mouse model was established by feeding a high-fat diet.Moxibustion was applied mildly at a distance of 3-5 cm from the"Zhongwan"(CV12)with a temperature maintained at(46±1)℃in the moxibustion group.In the sham control group,moxibustion was applied at a distance of 8-10 cm from CV12 with a temperature maintained at(38±1)℃.Changes in body weight and food intake were observed before and after treatment.Enzyme-linked immunosorbent assay(ELISA)was used to measure the levels of serum triglycerides(TG),total cholesterol(TC),and hypothalamic cAMP.The mass of abdominal white adipose tissue(WAT)was assessed.Histopathological changes in adipose tissue were observed using hematoxylin-eosin(HE)staining.Real-time fluorescence quantitative PCR and Western blot were employed to detect the mRNA and protein expression of UCP1,Prdm16,and PGC-1α in abdominal adipose tissue,as well as the expression of PKA and CREB in the hypothalamus.Results Compared with the control group,the model group showed increased body weight,food intake,serum TC and TG levels(P<0.01),as well as increased abdominal WAT mass(P<0.01),and significantly enlarged adipocyte volume in abdominal adipose tissue.Mice in the model group showed decreased mRNA and protein expression of UCP1,Prdm16,and PGC-1α in abdominal adipose tissue,as well as decreased mRNA and protein expression of PKA and CREB in the hypothalamus(P<0.01),along with decreased levels of hypothalamic cAMP(P<0.01).Compared with the model group and sham control group,the moxibustion group showed decreased body weight and food intake(P<0.01,P<0.05),decreased serum TC and TG levels,and abdominal WAT mass(P<0.01),along with reduced adipocyte diameter in abdominal adipose tissue.Moreover,compared with the model group and sham control group,the moxibustion group showed increased mRNA and protein expression of UCP1,Prdm16,and PGC-1α in abdominal adipose tissue,and the mRNA and protein expression of PKA and CREB in the hypothalamus(P<0.01),as well as increased levels of hypothalamic cAMP(P<0.01).Conclusion Moxibustion can enhance the mRNA and protein expression of UCP1,Prdm16,and PGC-1α in WAT of obese mice,improving obesity and lipid metabolism.Its mechanism may involve the regulation of the hypothalamic cAMP/PKA/CREB signaling pathway.关键词
肥胖/艾灸/下丘脑/脂肪组织Key words
Obesity/Moxibustion/Hypothalamus/Adipose tissue引用本文复制引用
傅成伟,陈霞,黄伟,韦丹,张艳佶,张英溶,周仲瑜..艾灸调控下丘脑cAMP/PKA/CREB信号通路对肥胖小鼠脂肪组织棕色化的影响[J].针刺研究,2025,50(10):1143-1151,9.基金项目
湖北省自然科学基金项目(No.2022CFD160) （No.2022CFD160）
国家中医药管理局"中医药古籍文献和特色技术传承专项"项目(No.GZY-KJS-2020-078) （No.GZY-KJS-2020-078）
武汉市科技局知识创新专项项目(No.2023020201010172) （No.2023020201010172）
国家中医药管理局高水平中医药重点学科建设项目(No.国中医药人教函[2023]85号) （No.国中医药人教函[2023]85号）
