针刺研究2025，Vol.50Issue(10)：1161-1168,8.DOI:10.13702/j.1000-0607.20240673
基于CaMKⅡ/RyR3通路探讨艾灸对阿尔茨海默病小鼠突触可塑性的影响
Effect of moxibustion on synaptic plasticity in mice with Alzheimer's disease based on the CaMKⅡ/RyR3 pathway
摘要
Abstract
Objective To explore the mechanism by which moxibustion promotes synaptic plasticity of hippocampal neurons and improves learning and memory in mice with Alzheimer's disease(AD)based on the calmodulin-dependent protein kinase Ⅱ(CaMKⅡ)/ryanodine receptor(RyR)3 pathway.Methods Forty APP/PS1 mice were randomly divided into the model(n=15),moxibustion(n=25)groups,and 15 C57BL/6J mice served as the blank control group.The mice in the moxibustion group were given moxibustion at the"Baihui"(GV20)and"Yongquan"(KI1)acupoints,30 minutes each time,once a day.A course of treatment lasted for 5 days,and there were a total of 4 courses of treatment.Ten mice from the moxibustion group were randomly selected as the moxibustion+inhibitor group,and were intraperitoneally injected with the CaMKⅡ protein inhibitor KN-93(2 mg/100 g)one day before sample collection on the basis of moxibustion.After the treatment,the shuttle box test was used to evaluate the learning and memory ability of the mice.Transmission electron microscopy was used to detect the changes in the ultrastructure of synapses in the CA1 region of the hippocampus.Western blot was used to detect the expression levels of β-amyloid protein(Aβ)and CaMKⅡ protein in the hippocampus of the mice.Immunofluorescence staining was used to detect the positive expression of CaMKⅡ in the hippocampus and the co-expression of RyR3/PSD95.Results Compared with the blank control group,the number of active avoidance responses,the protein expression and positive expression of CaMKⅡ in the hippocampus,and the percentage of the co-expression area of RyR3/PSD95 of the mice in the model group were decreased(P<0.01,P<0.05),and the number of synaptic vesicles in the CA1 region of the hippocampus was decreased;while the expression of Aβ protein in the hippocampus was increased(P<0.01).Compared with the model group,the number of active avoidance responses,the protein expression and positive expression of CaMKⅡ in the hippocampus,and the co-expression area percentage of RyR3/PSD95 of the mice in the moxibustion group were increased significantly(P<0.01),and the number of synaptic vesicles in the CA1 region of the hippocampus was increased;while the expression of Aβ protein was decreased(P<0.05).Compared with the moxibustion group,the number of active avoidance,the positive expression of CaMKⅡ and the co-expression area percentage of RyR3/PSD95 in the moxibustion+inhibitor group were decreased(P<0.01),and the number of synaptic vesicles in the CA1 region of the hippocampus was decreased.Conclusion Moxibustion can improve the learning and memory ability of AD model mice,and its mechanism may be related to up-regulating the expression of CaMKⅡ,activating the endoplasmic reticulum RyR3 channel,inducing the release of Ca2+,and then promoting neuronal synaptic plasticity.关键词
艾灸/阿尔茨海默病/突触可塑性/钙/钙调蛋白依赖性激酶ⅡKey words
Moxibustion/Alzheimer's disease/Synaptic plasticity/Calcium/calmodulin-dependent protein kinase Ⅱ引用本文复制引用
雷露,梅书雅,吕瑛,黄功琴,吕沛然,刘奇,张宁..基于CaMKⅡ/RyR3通路探讨艾灸对阿尔茨海默病小鼠突触可塑性的影响[J].针刺研究,2025,50(10):1161-1168,8.基金项目
陕西省自然科学基础研究计划项目(No.2022JQ-890) （No.2022JQ-890）
国家自然科学基金项目(No.82205284) （No.82205284）
