中国当代儿科杂志2025，Vol.27Issue(10)：1191-1198,8.DOI:10.7499/j.issn.1008-8830.2502073
靶向二代测序技术在新生儿呼吸窘迫综合征病原体检测中的价值:一项前瞻性随机对照研究
Value of targeted next-generation sequencing in pathogen detection for neonates with respiratory distress syndrome:a prospective randomized controlled trial
摘要
Abstract
Objective To investigate the application value of targeted next-generation sequencing(tNGS)in the etiological diagnosis of moderate to severe respiratory distress syndrome(RDS)in neonates.Methods A prospective randomized controlled trial was conducted,enrolling 81 term and late-preterm neonates with moderate to severe RDS admitted to Fujian Children's Hospital between December 2023 and December 2024.Patients were randomly assigned to the conventional microbiological test(CMT)group(n=42)or the tNGS group(n=39).For routine pathogen detection,bronchoalveolar lavage fluid was obtained via bronchoscopy,and lower respiratory tract specimens were collected via the endotracheal tube;all specimens underwent culture,and some specimens additionally underwent polymerase chain reaction or antigen testing.In the tNGS group,tNGS was performed in addition to routine pathogen detection on the same specimen types.The detection rate of pathogens,the detection rate of co-infections,and the duration of antibiotic use were compared between the two groups.Results The pathogen detection rate in the tNGS group(18/39,46%)was significantly higher than that in the CMT group(8/42,19%)(P=0.009).The co-infection detection rate was 13%(5/39)in the tNGS group,while no co-infections were identified in the CMT group(P=0.024).Regarding treatment,the duration of antibiotic use in the tNGS group was shorter than that in the CMT group[(12±4)days vs(15±5)days,P=0.003].Conclusions tNGS significantly improves the pathogen detection rate in neonates with moderate to severe RDS and offers advantages in the rapid identification of co-infections and reduction of antibiotic treatment duration,suggesting it has clinical utility and potential for wider adoption.关键词
新生儿呼吸窘迫综合征/靶向二代测序/支气管肺泡灌洗液/新生儿Key words
Neonatal respiratory distress syndrome/Targeted next-generation sequencing/Bronchoalveolar lavage fluid/Neonate引用本文复制引用
张海红,欧阳夏,刘仙萍,黄少如,林云峰..靶向二代测序技术在新生儿呼吸窘迫综合征病原体检测中的价值:一项前瞻性随机对照研究[J].中国当代儿科杂志,2025,27(10):1191-1198,8.基金项目
福建省临床重点专科建设项目(闽卫医政函[2023]1163号) （闽卫医政函[2023]1163号）
福建医科大学启航基金项目(2022QH1212). （2022QH1212）
