中国动物检疫2025，Vol.42Issue(10)：36-41,6.DOI:10.3969/j.issn.1005-944X.2025.10.006
对1例黄牛急性死亡病例的病原宏基因组检测及遗传进化分析
Metagenomic Detection and Genetic Evolution Analysis of Pathogens in a Case of Acute Death of Yellow Cattle
马烁 1傅国璋 2沈雪鹰 2孙兴南 2韩永川 3杜春红 4童贻刚5
作者信息
- 1. 华北理工大学,河北唐山 063210
- 2. 腾冲市动物疫病控制中心,云南腾冲 679100
- 3. 腾冲市界头镇畜牧兽医站,云南腾冲 679107
- 4. 云南省地方病防治所,云南大理 671000
- 5. 北京化工大学生命科学与技术学院,绿色生物制造国家重点实验室,北京 100029
- 折叠
摘要
Abstract
In order to identify causes leading to acute death of yellow cattle in Tengchong City of Yunnan Province from March 2023 to March 2024,tissue samples including heart,lung,brain,etc.were collected from one dead case for metagenomic next-generation sequencing(mNGS)after eliminating infection with relevant pathogens.Based on the mNGS results,primers for PCR amplification were designed,and the full-length genome sequence of the pathogen was obtained through PCR amplification,followed by phylogenetic analysis.The results revealed that the sequences of bovine adenovirus type 7(BAdV-7)were detected from the tissue samples,and one complete genome sequence of 30 006 bp(YN-BAdV7)was obtained through PCR amplification;the sequence was 99.6％identical to the nucleotide sequence of German reference strain(OM677816.1)isolated in 2022 and U.S.reference strain(MN901942.2)in 2021,and clustered within the same evolutionary branch as the strains prevalent in Europe and America,obviously different from other types of adenoviruses.In conclusion,BAdV-7 was prevalent in Tengchong City,closely and genetically linking to the strains prevalent in Europe and America,which was likely responsible for the acute death of local cattle.It was thereby recommended to reduce farmers' losses by improving traceability,prevention and control against its infection.关键词
宏基因组学检测/牛腺病毒7型/遗传进化分析/黄牛Key words
metagenomic detection/BAdV-7/genetic evolution analysis/yellow cattle分类
农业科技引用本文复制引用
马烁,傅国璋,沈雪鹰,孙兴南,韩永川,杜春红,童贻刚..对1例黄牛急性死亡病例的病原宏基因组检测及遗传进化分析[J].中国动物检疫,2025,42(10):36-41,6.
