中国免疫学杂志2025，Vol.41Issue(10)：2313-2317,5.DOI:10.3969/j.issn.1000-484X.2025.10.002
Tfh细胞及其相关分子在早发性卵巢功能不全小鼠模型中的变化研究
Study on changes of Tfh cells and their related molecules in mouse model of premature ovarian insufficiency
摘要
Abstract
Objective:Haracterization of follicular helper T cells(Tfh)cells and related molecules early onset in premature ovarian insufficiency(POI)based on a mouse model of POI.Methods:After acclimatization feeding of 30 BALB/c mice were selected,mice were subjected to vaginal exfoliative cytology from 08:00 onwards every day for 10 consecutive days under light microscope obser-vation,20 healthy female mice with regular estrous cycle were screened out and randomly divided into model(POI)group and healthy control(HC)group according to random number table method,different chemical interventions were given and processed and tissue sampling was done on the 21st day.Every morning from the beginning to the end of the modeling period,the mice were subjected to cell smears of vaginal secretions,which were stained with Giemsa's stain and then observed under a light microscope.After the mice were sacrificed,bilateral ovarian tissues were taken and ovarian paraffin sections were made,and the tissues were stained with HE to observe pathological changes in ovaries of mice.Immunohistochemistry was used to detect expressions of ovarian Tfh-related molecules CXCR5 and BCL-6;flow assay was used to detect frequency of CD4+CXCR5+Tfh cells and CD4+ICOS+Tfh cells in spleens of mice;ELISA was used to detect levels of IL-6 and IL-21 in splenic tissues of mice;qRT-PCR was used to detect mRNA levels of CXCR5 and BCL-6 in mice;correlation analysis was performed to correlate frequencies of CD4+CXCR5+Tfh and CD4+ICOS+Tfh cells with levels of IL-6 and IL-21 in spleens of mice in both groups.Results:Pathological changes in ovarian tissue of mice in POI group:primordial folli-cles and growing follicles decreased,while atretic follicles increased.BCL-6 and CXCR5 were highly expressed in ovarian tissue of mice in POI group.Frequency of CD4+CXCR5+Tfh and CD4+ICOS+Tfh cells in spleen of POI mice was higher than that in HC group.Levels of IL-6 and IL-21 in spleens of mice in POI group were significantly higher than those in HC group,and were positively correlated with the frequency of CD4+CXCR5+Tfh and CD4+ICOS+Tfh cells in spleen of mice.Conclusion:In POI mouse model,Tfh cells are highly expressed and the level of Tfh-related molecules changes,which provides basic reference for studying pathogenesis of POI.关键词
早发性卵巢功能不全/Tfh细胞/动物模型Key words
Premature ovarian insufficiency/Follicular helper T cells/Animal model分类
医药卫生引用本文复制引用
麦尔哈巴·艾斯卡尔,朱玥洁,张曼丽,张于念,王辉,巩晓芸,丁剑冰..Tfh细胞及其相关分子在早发性卵巢功能不全小鼠模型中的变化研究[J].中国免疫学杂志,2025,41(10):2313-2317,5.基金项目
省部共建国家重点实验室开放课题(SKL-HIDCA-2022-JZ6) （SKL-HIDCA-2022-JZ6）
河南省免疫与靶向药物重点实验室开放课题(HNKLITD2022001). （HNKLITD2022001）
