中国免疫学杂志2025，Vol.41Issue(10)：2318-2323,6.DOI:10.3969/j.issn.1000-484X.2025.10.003
青蒿琥酯调节RhoA/ROCK信号通路对高糖诱导的滋养层细胞损伤的影响
Effect of artesunate on high glucose induced trophoblast cell damage by regulating RhoA/ROCK signaling pathway
摘要
Abstract
Objective:To investigate effect of artesunate(ART)on high glucose(HG)induced trophoblast cell damage and its mechanism.Methods:Human trophoblast cells HTR-8/SVneo were separated into control group,HG group,ART low concentra-tion group,ART high concentration group and ART high concentration+lysophosphatidic acid(LPA)group.CCK-8 method was applied to detect cell viability.Flow cytometry was applied to detect cell apoptosis.ELISA was applied to detect levels of TNF-α,IL-1β and IL-10.Reagent kits were used to detect levels of lactate dehydrogenase(LDH),malondialdehyde(MDA)and superoxide dismutase(SOD).Western blot was applied to detect expressions of Bax,Bcl-2,Ras homolog gene family member A(RhoA),Rho associated coiled coil containing protein kinase(ROCK)1 and ROCK2.Results:After treatment with 0.4～1.6 μmol/L ART,activity of HTR-8/SVneo cells were increased(P<0.05).ART greatly enhanced activity of HTR-8/SVneo cells induced by HG,and cell viability was most significantly increased under 1.6 μmol/L ART treatment(P<0.05).Compared with control group,activity of HTR-8/SVneo cells,IL-10,SOD and Bcl-2 levels were reduced in HG group,apoptosis rate,TNF-α,IL-1β,LDH,MDA,Bcl-2,RhoA,ROCK1 and ROCK2 levels were increased(P<0.05).Compared with HG group,activity of HTR-8/SVneo cells,IL-10,SOD and Bcl-2 levels were increased in ART low and high concentration groups,apoptosis rate,TNF-α,IL-1β,LDH,MDA,Bcl-2,RhoA,ROCK1 and ROCK2 levels were decreased(P<0.05).Compared with ART high concentration group,activity of HTR-8/SVneo cells,IL-10,SOD and Bcl-2 levels were decreased in ART high concentration+LPA group,apoptosis rate,TNF-α,IL-1β,LDH,MDA,Bcl-2,RhoA,ROCK1 and ROCK2 levels were increased(P<0.05).Conclusion:ART can inhibit inflammatory response and oxidative stress of trophoblast cells and reduce cell apoptosis,whose mechanism may be related to inhibition of RhoA/ROCK signaling pathway.关键词
滋养层细胞/高糖/青蒿琥酯/RhoA/ROCK信号通路Key words
Trophoblast cells/High glucose/Artesunate/RhoA/ROCK signaling pathway分类
医药卫生引用本文复制引用
吴晓茜,朱燕,徐淋,刘艳,李敏..青蒿琥酯调节RhoA/ROCK信号通路对高糖诱导的滋养层细胞损伤的影响[J].中国免疫学杂志,2025,41(10):2318-2323,6.基金项目
国家儿童健康与疾病临床医学研究中心临床医学研究一般项目(NCRCCHD-2022-GP-0X). （NCRCCHD-2022-GP-0X）
