中国免疫学杂志2025，Vol.41Issue(10)：2329-2333,5.DOI:10.3969/j.issn.1000-484X.2025.10.005
p38MAPK信号通路介导孕酮下调蜕膜基质细胞分泌IL-8对早期自然流产的影响
Effect of p38MAPK signaling pathway mediating progesterone down-regulation of IL-8 secretion by decidual stromal cells on early spontaneous miscarriage
摘要
Abstract
Objective:To investigate the effect of p38MAPK signaling pathway mediating progesterone regulation on IL-8 protein secretion by decidual stromal cells(DSCs)on early spontaneous miscarriage.Methods:IHC and Western blot were applied to detect protein expressions of p38MAPK and p-p38MAPK in decidual tissues of miscarriage group and control group.Human DSCs in early pregnancy were isolated in vitro and cultured to be treated with different concentrations of progesterone(0.01 μmol/L,0.1 μmol/L,1 μmol/L and 10 μmol/L),with ELISA measuring IL-8 protein secretion from DSCs and Western blot measuring protein expressions of p38MAPK and p-p38MAPK in DSCs.After treatment with p38MAPK inhibitor SB203580,IL-8 protein secretion was detected by ELISA in progesterone+inhibitor group,progesterone group and control group.Results:Protein expression of p-p38MAPK in decidual tissues of miscarriage group was significantly higher than that of control group(P=0.002 3).Protein secretion of IL-8 in DSCs of 0.01 μmol/L progesterone group was lower than that of control group(P=0.027 6),protein expression of p-p38MAPK in DSCs of 0.1 μmol/L proges-terone group was lower than that of control group(P=0.025 3),IL-8 protein expression was significantly lower than that in control group(P=0.007 0),and protein expressions of both IL-8 and p-p38MAPK from DSCs in 1 μmol/L and 10 μmol/L progesterone groups were significantly lower than those in control group(P=0.003 2,P=0.001 9;P=0.002 2,P=0.001 3).IL-8 protein secretion in proges-terone+p38MAPK inhibitor group was further reduced compared to progesterone group(P=0.046 6).Conclusion:Abnormal activation of p38MAPK signaling pathway is involved in early spontaneous miscarriage,and progesterone may down-regulate IL-8 expression in DSCs by inhibiting p38MAPK phosphorylation to correct early spontaneous miscarriage caused by Th1/Th2 cytokine imbalance.关键词
蜕膜基质细胞/孕酮/白细胞介素-8/p38MAPK信号通路/早期自然流产Key words
Decidual stromal cells/Progesterone/IL-8/p38MAPK signaling pathway/Early spontaneous miscarriage分类
医药卫生引用本文复制引用
梁诗维,栾兆进,马铭艳,宫晓玲,赵紫薇,宋芳,杨美霞..p38MAPK信号通路介导孕酮下调蜕膜基质细胞分泌IL-8对早期自然流产的影响[J].中国免疫学杂志,2025,41(10):2329-2333,5.基金项目
内蒙古自然科学基金项目(2021MS08056) （2021MS08056）
内蒙古自治区卫生健康科技计划项目(202201393) （202201393）
包头医学院博士启动基金项目(BSJJ202205). （BSJJ202205）
