中国免疫学杂志2025，Vol.41Issue(10)：2341-2347,2353,8.DOI:10.3969/j.issn.1000-484X.2025.10.007
高级别浆液性卵巢癌患者新辅助化疗前后肿瘤浸润淋巴细胞差异与预后的关系
Analysis of tumor infiltrating lymphocytes changes after neoadjuvant chemotherapy with prognosis in high-grade serous ovarian cancer
摘要
Abstract
Objective:To investigate expression changes of tumor infiltrating lymphocytes(TILs)and their subtypes CD4+TILs,CD8+TILs,CD20+TILs and Foxp3+TILs in paired tumor samples pre-and post-neoadjuvant chemotherapy(NACT)in high-grade serous ovarian cancer(HGSOC)patients and their association with chemotherapy sensitivity and prognosis.Methods:A total of 40 patients with HGSOC who underwent NACT and interval debulking surgery(IDS)at Fourth Hospital of Hebei Medical University from August 2017 to February 2023 were retrospectively analyzed.CD4+TILs,CD8+TILs,CD20+TILs and Foxp3+TILs expressions in paired tumor samples pre-and post-NACT were detected by immunohistochemical staining.Divided patients into two groups based on KELIM score and platinum sensitivity,expressions of TILs,CD4+TILs,CD8+TILs,CD20+TILs and Foxp3+TILs were compared be-tween two groups,and their correlation with prognosis was analyzed.Results:Density of TILs(P=0.003,P=0.01),CD8+TILs(P<0.001,P=0.014)and CD20+TILs(P<0.001,P=0.003)post-NACT in patients with KELIM scores≥1 and platinum sensitivity were in-creased compared with pre-NACT.In contrast,there was no significant change in density of TILs pre-and post-NACT in patients with KELIM scores<1 and platinum resistance.Patients with high expression of CD8+TILs pre-NACT had longer progression free survival(PFS)and overall survival(OS)than patients with lower expression(P<0.001,P=0.011);patients with high expression of TILs post-NACT showed prolonged median PFS and OS(P<0.001,P=0.009);patients with high expression of CD20+TILs post-NACT showed prolonged median PFS(P=0.005).In multivariate analysis,density of CD8+TILs pre-NACT(HR=0.309,P=0.032),density of TILs post-NACT(HR=0.192,P=0.014),age(HR=2.962,P=0.036)were independent prognostic factors for PFS.Conclusion:Patients with HGSOC who have increased TILs density after NACT are more likely to be platinum sensitive.Expression of TILs,CD8+TILs and CD20+TILs in tumor site can serve as effective prognostic factors in HGSOC patients.关键词
高级别浆液性卵巢癌/新辅助化疗/肿瘤浸润淋巴细胞/KELIM评分/铂敏感性Key words
High-grade serous ovarian cancer/Neoadjuvant chemotherapy/Tumor infiltrating lymphocytes/KELIM score/Platinum sensitivity分类
医药卫生引用本文复制引用
卢立杰,刘式浩,刘月平,范元春,张辉..高级别浆液性卵巢癌患者新辅助化疗前后肿瘤浸润淋巴细胞差异与预后的关系[J].中国免疫学杂志,2025,41(10):2341-2347,2353,8.基金项目
河北省卫生健康委医学科学研究课题计划项目(20221344) （20221344）
河北省科技厅引才引智创新平台项目 （）
河北省财政厅老年病防治项目. （）
