中国农业科学2025，Vol.58Issue(19)：3825-3836,12.DOI:10.3864/j.issn.0578-1752.2025.19.003
111份紫花苜蓿种质资源苗期表型抗旱性鉴定评价
Evaluation of 111 Alfalfa Germplasm Resources for Seedling Phenotypic Drought Tolerance Characterization
摘要
Abstract
[Objective]To explore the ideal traits and methods that can characterize the drought resistance of the core germplasm population of alfalfa at the seedling stage,and to mine excellent drought-resistant germplasm,so as to provide technical support and material basis for the identification of drought resistance of alfalfa at the seedling stage,germplasm innovation and breeding.[Method]Using 111 core germplasms of alfalfa as materials,the single drought resistance coefficients of six traits,namely plant height(PH),shoot fresh weight(SFW),shoot dry weight(SDW),main root length(MRL),root fresh weight(RFW),and root dry weight(RDW),were determined under natural drought stress and normal water supply(control).Combining the analysis methods such as the single drought resistance coefficient,correlation,principal component,membership function,linear stepwise regression,and clustering of each trait of alfalfa,the response differences of each alfalfa germplasm to drought stress were evaluated,the traits and methods for identifying drought resistance at the seedling stage were screened,and the excellent resources of drought resistance at the seedling stage of alfalfa were explored.[Result]The analysis of the single drought resistance coefficient indicated that drought stress significantly inhibited all six traits of alfalfa at the seedling stage.The results of trait correlation showed that there was a positive correlation among the six traits.The principal component results indicated that the six individual drought-resistant traits of alfalfa could be combined into four comprehensive drought-resistant traits,and the cumulative contribution rate reached 86.885%.The methods such as single drought resistance coefficient,correlation,principal component and linear stepwise regression were comprehensively evaluated to determine that SFW,SDW,RFW and RDW were the ideal traits for evaluating alfalfa materials at the seedling stage.Using the classification methods of CDC value and D value,the drought resistance of 111 core germplasm populations of alfalfa at the seedling stage was classified.It was found that there were only subtle differences in the classification of drought resistance of various germplasm by the two drought resistance classification methods.The D values of 111 alfalfa core germplasms were clustered and classified.The various germplasms were divided into 5 categories,which belonged to strongly drought-resistant,moderately drought-resistant,moderately drought-resistant and drought-sensitive germplasms respectively.[Conclusion]Determine that SFW,SDW,RFW and RDW are the best traits for drought resistance evaluation of alfalfa at the seedling stage;The number of highly drought-resistant and relatively drought-resistant materials of alfalfa at the seedling stage excavated was 6 and 25 respectively.关键词
紫花苜蓿/种质资源/苗期/干旱胁迫/综合评价Key words
alfalfa/germplasm resources/seedling stage/drought stress/comprehensive assessment引用本文复制引用
包明芳,秦燕,陈彩锦,张尚沛,张国辉,沙晓弟..111份紫花苜蓿种质资源苗期表型抗旱性鉴定评价[J].中国农业科学,2025,58(19):3825-3836,12.基金项目
固原市创新驱动科技研发"拨改投"项目(2024BGTYF01-33)、宁夏回族自治区重点研发计划(2022BBF02029)、宁夏农业种质资源库基础条件建设(2022DPC05001)、宁夏生态农业产业专家服务基地(2420000001) （2024BGTYF01-33）
