围绝经期干眼肝肾阴虚证大鼠模型的建立与评价
Establishment and evaluation of a rat model of perimenopausal dry eye with liver-kidney yin deficiency evidence
摘要
Abstract
Objective To establish a rat model of perimenopausal dry eye with liver and kidney yin deficiency from the perspective of"measuring evidence by prescription"in Chinese medicine.Methods Thirty SPF-grade female SD rats were divided randomly into sham-operated,model,and Qiju Dihuang soup groups(n=10).Rats in the latter two groups underwent bilateral ovariectomy and were given 0.1％benzalkonium chloride eye drops combined with provocation for 10 weeks to establish a model of perimenopausal dry eye with liver and kidney yin deficiency.After modeling,rats in the Qiju Dihuang soup group were gavaged with Qiju Dihuang soup at a dose of 8.37 g/(kg·d),rats in the sham-operated and model groups were gavaged with saline at a rate of 1 mL/100(g·d)for 21 d.The general condition,retinal screen test scores,tear secretion,time of tear film rupture,and corneal fluorescein staining were observed and recorded in each group.Serum levels of follicle-stimulating hormone(FSH),estradiol(E2),and progesterone(PROG)were measured by enzyme-linked immunosorbent assay.Pathological damage to the cornea and lacrimal glands were detected by hematoxylin/eosin staining.Expression levels of the inflammatory factors interleukin(IL)-1β and tumor necrosis factor(TNF)-a in the cornea were detected by immunohistochemistry.Results Compared with the sham-operated group,rats in the model group showed behavioral signs of slow action and reaction,irritability,body mass loss,and increased anal temperature(P＜0.05),decreased retinal screen test scores(P＜0.05),tear secretion(P＜0.05),and time of tear film rupture(P＜0.05),and pathological damage to the cornea and lacrimal glands.FSH levels increased and E2 and PROG levels decreased(P＜0.05)and expression levels of IL-1 and TNF-α increased in the model group compared with the sham group.All the above indexes were significantly improved in the Qiju Dihuang soup group compared with the model group.Conclusions From the perspective of"measuring evidence by formula"in Chinese medicine,we successfully established a rat model of perimenopausal dry eye with liver-kidney yin deficiency syndrome,which provides a theoretical and experimental basis for future systematic and in-depth research on the mechanism of perimenopausal dry eye with formula and evidence.关键词
干眼/围绝经期/肝肾阴虚证/动物模型Key words
dry eye/perimenopause/liver-kidney yin deficiency syndrome/animal model分类
生物科学引用本文复制引用
田赛男,刘桂成,陈瑶,刘培,彭俊,彭清华,胡淑娟..围绝经期干眼肝肾阴虚证大鼠模型的建立与评价[J].中国实验动物学报,2025,33(9):1270-1279,10.基金项目
国家自然科学基金项目(81574031),国家中医药管理局人才支持项目-岐黄学者(国中医药人教函[2022]6号),湖南省人才托举工程-年青优秀科技人才培养计划(2023TJ-N25),湖南省科技厅临床创新引导项目(2021SK51402),湖南省教育厅重点项目(22A0241,23A0300),湖南省中医药科研课题重点课题(A2023001),湖南省卫生健康委卫生科研课题一般课题(W20243198),湖南中医药大学校院联合基金重点项目(2023XYLHJJ005),湖南中医药大学校级科研项目一般项目(Z2023XJYB21),湖南中医药大学研究生创新课题项目(2024CX155).Funded by National Natural Science Foundation of China Project(81574031),Talent Support Project of the National Administration of Traditional Chinese Medicine-Qi Huang Scholar(National Chinese Medicine Education Letter[2022]No.6),Young Excellent Science and Technology Talent Cultivation Program of Hunan Province's Talent Support Project(2023TJ-N25),Hunan Provincial Science and Technology Department Clinical Innovation Guidance Project(2021SK51402),Key Projects of the Education Department of Hunan Province(22A0241,23A0300),Key Project of Traditional Chinese Medicine Scientific Research in Hunan Province(A2023001),General Project of Health Research Program,Hunan Provincial Health Commission(W20243198),Key Project of the University-Hospital Joint Fund of Hunan University of Chinese Medicine(2023XYLHJJ005),General Project of University-Level Scientific Research Program of Hunan University of Chinese Medicine(Z2023XJYB21),Graduate Innovation Project of Hunan University of Chinese Medicine(2024CX155). （81574031）
