中国兽医杂志2025，Vol.61Issue(10)：33-41,9.DOI:10.20157/j.cnki.zgsyzz.2025.10.005
湖南省中部和西部地区山羊源肝片吸虫的遗传多样性和遗传进化分析
Genetic Diversity and Evolutionary Analysis of Fasciola hepatica Collected from Goats in the Central and Western Regions of Hunan Province
摘要
Abstract
To explore the genetic diversity of Fasciola hepatica(F.hepatica)isolated from goats in central and western Hunan Province and to analyze the characteristics of their ribosomal and mitochondrial gene sequences as well as their genetic evolutionary relationships,this study examined 36 goat-derived F.hepatica isolates from Zhangjiajie,Xiangxi,Huaihua,Shaoyang,Yongzhou,and Loudi.PCR amplification and sequencing were performed for five genetic loci:18S ribosomal RNA(18S rRNA),internal transcribed spacer 1(ITS-1),internal transcribed spacer 2(ITS-2),mitochondrial cytochrome C oxidase subunit I(cox1),and NADH dehydrogenase subunit I(nad1).Sequence characteristics,intraspecific and interspecific differences,and phylogenetic relationships were analyzed.The results showed that the sequence lengths for 18S rRNA,ITS-1,ITS-2,cox1,and nad1 genes were 585,453,360,676,and 903 bp,respectively.Among these,18S rRNA and ITS-1 exhibited a GC base preference,while ITS-2,cox1,and nad1 were AT-rich.The intraspecific variations for these genes were 0-1.0%,0-2.0%,0-0.3%,0-2.7%,and 0-1.7%,respectively,while interspecific differences ranged from 0.68%-1.85%,0.44%-3.52%,1.11%-9.44%,1.48%-13.46%,and 8.31%-14.51%,respectively.Phylogenetic analysis revealed that all 36 isolates clustered on the same branch,indicating that despite low levels of genetic diversity among goat-derived F.hepatica in these regions,the isolates share close genetic relationships.Additionally,ITS-2 and nad1 genes were identified as useful genetic markers for interspecific studies of Fasciola species.关键词
肝片吸虫/序列特征/种内差异/种间差异/遗传进化关系Key words
Fasciola hepatica/sequence characteristics/intraspecific variation/interspecific variation/phylogenetic rela-tionship分类
农业科技引用本文复制引用
苏五珍,李中波,刘伟..湖南省中部和西部地区山羊源肝片吸虫的遗传多样性和遗传进化分析[J].中国兽医杂志,2025,61(10):33-41,9.基金项目
湖南省怀化市科技局项目(2023R2208) （2023R2208）
湖南省教育厅优秀青年项目(23B1072) （23B1072）
湖南省自然科学基金项目(2022JJ50319) （2022JJ50319）
动物源性人兽共患病防控福建省高校工程研究中心项目(2022K004) （2022K004）
