中国兽医杂志2025，Vol.61Issue(10)：64-70,7.DOI:10.20157/j.cnki.zgsyzz.2025.10.009
经饮水和滴鼻点眼途径接种新城疫病毒弱毒株La Sota的感染性比较
Comparison of the Infectivity of Newcastle Disease Virus Lentogenic Strain La Sota in Chickens Via the Drinking Water or Ocular-nasal Route
摘要
Abstract
To compare the infectivity of the Newcastle disease virus(NDV)La Sota strain administered through different routes in chickens,this study first identified the biological characteristics of the La Sota strain,then evaluated the virus's tissue tropism,viral load,shedding,and host antibody responses following administration via drinking water or ocular-nasal routes.The results showed that neither route of administration caused clinical symptoms or pathological changes in 2-week-old specific pathogen free(SPF)chickens.The virus replicated in multiple organs with viral loads ranging from 101.2 to 103.4 copies/μg.No virus shedding was detected from either the respiratory or digestive tracts.On day 7 post-inoculation,hemagglutination inhibition(HI)antibody titers in the ocular-nasal and drinking water groups were 27.2 and 24.2,respectively,with a significant difference(P<0.05).On day 14,titers were 26.2 and 27.8,with no significant difference(P>0.05).These findings indicate that La Sota strain can replicate in multiple tissues when administered through both routes,does not shed through respiratory or digestive tracts,and induces robust HI antibody responses.This study provides a reference for selecting appropriate delivery routes of live attenuated NDV vaccines in clinical settings.关键词
新城疫病毒/La Sota/接种途径/感染性/组织嗜性/抗体应答Key words
Newcastle disease virus/La Sota/inoculation route/infectivity/tissue tropism/antibody response分类
农业科技引用本文复制引用
王蔼甜,何晓政,柴宇昊,刘心怡,胡增垒..经饮水和滴鼻点眼途径接种新城疫病毒弱毒株La Sota的感染性比较[J].中国兽医杂志,2025,61(10):64-70,7.基金项目
国家自然科学基金面上项目(32372977) （32372977）
江苏省高等学校基础科学(自然科学)研究重大项目(24KJA230004) （自然科学）
教育部农业与农产品安全国际合作联合实验室开放课题(JILAR-KF202203) （JILAR-KF202203）
扬州大学高端人才成长计划项目 （）
