中国畜牧兽医2025，Vol.52Issue(10)：4592-4602,11.DOI:10.16431/j.cnki.1671-7236.2025.10.006
亚临床酮病奶牛血清脂类代谢物的变化特征分析
Characteristics of Serum Lipid Metabolites in Diary Cows with Subclinical Ketosis
摘要
Abstract
[Objective]The objective of this study was to compare the differences in serum lipid metabolites between healthy and subclinical ketosis diary cows at 14 d after parturition,and to investigate the characteristics of changes in serum lipid metabolism in subclinical ketosis diary cows during the early lactation period and the relationship with subclinical ketosis.[Method]Blood samples were collected from 70 periparturient cows,blood biochemical indices were measured.According to the concentration of β-hydroxybutyrate(BHBA)in serum,10 healthy dairy cows(H group)and 10 subclinical ketosis diary cows(SCK group)were selected respectively.Liquid chromatography-mass spectrometry(LC-MS)technology was used to perform metabolomic analysis of serum from diary cows in H and SCK groups at 14 d postpartum.Differential metabolites of serum lipids were screened and KEGG pathway enrichment analysis was performed on them,and evaluate its correlation with the blood biochemical indicators related to ketosis.[Result]Compared with healthy dairy cows,the differential metabolites that showed significant changes in the serum of subclinical ketosis dairy cows were mainly fatty acylates,glycerophospholipids and glyceride.These metabolites were significantly correlated with the blood biochemical indicators related to ketosis,such as non-esterified fatty acids,β-hydroxybutyric acid,direct bilirubin,total bilirubin,glucose,total cholesterol concentrations,and aspartate aminotransferase activity(P＜0.05).The differential metabolites of 14 serum lipids,including lysophosphatidylcholine(18∶0/0∶0),leukotrienes,5,6-dihydroxy-8Z,11Z,14Z-eicosapentaenoic acid,succinic semialdehyde,stearic acid,oleic acid,etc.,were mainly enriched in pathways such as ether lipid metabolism,arachidonic acid metabolism,alanine-aspartic acid and glutamic acid metabolism.[Conclusion]The composition of serum lipid metabolites in subclinical ketosis dairy cows in the early lactation period had undergone significant changes.Multiple metabolic pathways in cows,such as ether lipid metabolism,arachidonic acid metabolism,unsaturated fatty acid biosynthesis,butyric acid metabolism,fatty acid biosynthesis,and glycerophospholipid metabolism,were affected.The disorder of serum lipid metabolites in lactating cows was closely related to the occurrence of subclinical ketosis.关键词
奶牛/亚临床酮病/血清/脂质代谢Key words
diary cow/subclinical ketosis/serum/lipid metabolism分类
农业科技引用本文复制引用
李欢语,张万里,王爽,崔省委,马燕芬,马云,余永涛..亚临床酮病奶牛血清脂类代谢物的变化特征分析[J].中国畜牧兽医,2025,52(10):4592-4602,11.基金项目
宁夏自然科学基金项目(2023AAC03051) （2023AAC03051）
宁夏回族自治区重点研发计划项目(2024BBF02018、2021BEF01001) （2024BBF02018、2021BEF01001）
