中国畜牧兽医2025，Vol.52Issue(10)：4603-4613,11.DOI:10.16431/j.cnki.1671-7236.2025.10.007
亚硒酸钠和硒代蛋氨酸对马骨骼肌卫星细胞氧化损伤的缓解作用及线粒体功能的影响
Effects of Sodium Selenite and Selenomethionine on the Mitigation of Oxidative Damage and Mitochondrial Function in Equine Skeletal Muscle Satellite Cells
摘要
Abstract
[Objective]This study aimed to investigate the effects of different concentrations of sodium selenite and selenomethionine on H2O2-induced oxidative damage and mitochondrial function in horse skeletal muscle satellite cells,and provide a reference for selenium as an antioxidant to alleviate oxidative stress during exercise in horses.[Method]In this study,the oxidative stress model of horse skeletal muscle satellite cells was induced by 600 μmol/L H2O2.After 6 h of starvation medium culture,different concentrations of sodium selenite(10,20,50,100,150 and 200 nmol/L)and selenomethionine(10,20,50,100,150 and 200 nmol/L)were used to culture for 24 h.Then,horse skeletal muscle satellite cells were induced with 600 μmol/L H2O2 for 6 h.Finally,samples were collected.CCK-8 kit was used to detect the cell viability,antioxidant enzyme kit was used to detect the indices of antioxidant enzyme activity,Real-time quantitative PCR was used to detect the expression of antioxidant genes,and mitochondrial stress kit was used to detect the mitochondrial energy metabolism of skeletal muscle satellite cells to evaluate the mitigation effect of different concentrations of sodium selenite and selenomethionine on oxidative damage.[Result]The relative cell viability of 100 nmol/L sodium selenite group and 150 nmol/L selenomethionine group was significantly higher than that of injury group(P＜0.05),there was no significant difference compared with control group(P＞0.05),the total antioxidant capacity(T-AOC)and antioxidant enzyme activity were significantly higher than those of injury and control groups(P＜0.05).The content of malondialdehyde(MDA)in cells decreased first and then increased with the increase of sodium selenite concentration,it was the lowest in 100 nmol/L sodium selenite group,which was significantly lower than those in control and injury groups(P＜0.05).The content of MDA in 150 nmol/L selenomethionine group was significantly lower than that in control and injury groups(P＜0.05).The expressions of RELA,glutathione peroxidase 1(GPX1),superoxide dismutase 1(SOD1)genes in 100 nmol/L sodium selenite and 150 nmol/L selenomethione groups were extremely significantly higher than those in control and injury groups(P＜0.01),the expression of thioredoxin reductase 1(TRXR1)gene was extremely significantly higher than that in injury group(P＜0.01),and the expression of TRXR1 gene in 150 nmol/L selenomethione group was extremely significantly higher than that in control group(P＜0.01).The expression of nuclear factor E2-related factor 2(Nrf2)gene in 150 nmol/L selenomethionine group was significantly higher than that in injury group(P＜0.05).The expression of RELA,GPX1,TRXR1 and SOD1 genes was extremely significantly higher than that in control group(P＜0.01).The basal respiration value,ATP oxygen consumption value and maximum respiration value were 32.28,21.70 and 45.01 pmol/min,respectively,which were significantly higher than those in injury group(P＜0.05),increased by 25.03％,30.22％and 25.59％,respectively.However,there was no significant difference in oxygen consumption rate and mitochondrial function parameters between 100 nmol/L sodium selenite group and injury group(P＞0.05).[Conclusion]Sodium selenite and selenomethionine could up-regulate the expression of antioxidant genes by activating the downstream signaling pathway of Nrf2,thereby increasing the activities of superoxide dismutase(SOD),catalase(CAT)and glutathione peroxidase(GPX),reducing MDA production and alleviating oxidative damage.Under this test condition,100 nmol/L sodium selenite and 150 nmol/L selenomethionine had the best remission effect.In addition,compared with the inorganic sodium selenite,organic selenomethionine could also improve mitochondrial energy metabolism.关键词
马/骨骼肌卫星细胞/亚硒酸钠/硒代蛋氨酸/氧化损伤Key words
horse/skeletal muscle satellite cells/sodium selenite/selenomethionine/oxidative damage分类
农业科技引用本文复制引用
张心壮,刘雨蒙,高星,王晓阳,尹艺旋,芒来..亚硒酸钠和硒代蛋氨酸对马骨骼肌卫星细胞氧化损伤的缓解作用及线粒体功能的影响[J].中国畜牧兽医,2025,52(10):4603-4613,11.基金项目
内蒙古农业大学优秀青年科学基金培育项目(BR230405) （BR230405）
内蒙古自治区重点研发项目(2023YFD0002) （2023YFD0002）
内蒙古自治区教育厅一流学科科研专项(YLXKZX-NND-007) （YLXKZX-NND-007）
内蒙古农业大学动物科学学院高水平成果培育专项(CG202421) （CG202421）
