中华中医药杂志2025，Vol.40Issue(8)：4035-4040,6.
活血潜阳祛痰方改善肥胖高血压大鼠心肌细胞焦亡的机制研究
Mechanistic study on Huoxue Qianyang Qutan Recipe in alleviating cardiomyocyte pyroptosis in obesity hypertension
摘要
Abstract
Objective:To observe the effects and potential mechanisms of the Huoxue Qianyang Qutan Recipe(HQQR)on cardiomyocyte pyroptosis in obesity hypertension(OBH).Methods:An OBH rat model was established by feeding spontaneously hypertensive rats(SHR)with a high-fat diet.Successfully modeled OBH rats were randomly divided into the model group(OBH),valsartan group(Valsartan),low-dose HQQR group[HQQR(L)],and high-dose HQQR group[HQQR(H)].Drug interventions were administered for 10 weeks.WKY and SHR rats served as the control groups.Primary cardiomyocytes(CMs)were isolated and cultured from SD rats and treated with angiotensin Ⅱ(Ang Ⅱ)to establish a CMs injury model.The CMs were divided into the control group,Ang Ⅱ group,low-dose HQQR group[Ang Ⅱ+HQQR(L)],high-dose HQQR group[Ang Ⅱ+HQQR(H)],and valsartan group[Ang Ⅱ+Valsartan].ELISA was used to measure IL-1β and IL-18 levels in tissues and cell supernatants.Cell viability was assessed using the CCK-8 assay.Western blot was employed to detect the protein expression of NOD-like receptor protein 3(NLRP3),Caspase 1/p10,and the N-terminal domain of gasdermin D(GSDMD-N).Statistical analysis was performed on the related data.Results:In OBH rat myocardial tissues,IL-1β and IL-18 levels were significantly elevated(P＜0.001),and the expression of NLRP3,Caspase 1/P10,and GSDMD-N proteins was significantly increased(P＜0.001).HQQR treatment significantly reduced myocardial IL-1β and IL-18 levels(P＜0.001)and decreased NLRP3,Caspase 1/P10,and GSDMD-N protein expression(P＜0.00l).Ang Ⅱ stimulation significantly reduced CMs viability,increased IL-1β and IL-18 levels in cell supernatants compared to the blank group(P＜0.001),and elevated NLRP3,Caspase 1/P10,and GSDMD-N protein expression(P＜0.001).HQQR intervention improved CMs viability(P＜0.05),reduced IL-1β and IL-18 levels(P＜0.001),and significantly decreased NLRP3,Caspase 1/P10,and GSDMD-N protein expression(P＜0.001).Conclusion:HQQR may improve CMs injury by inhibiting inflammation and reducing pyroptosis.关键词
活血潜阳祛痰方/肥胖/高血压/心肌细胞/焦亡Key words
Huoxue Qianyang Qutan Recipe(HQQR)/Obesity/Hypertension/Cardiomyocytes/Pyroptosis引用本文复制引用
马玉龙,芦波,周训杰,桂明泰,姚磊,李建华,王明珠,陈晓喆,符德玉..活血潜阳祛痰方改善肥胖高血压大鼠心肌细胞焦亡的机制研究[J].中华中医药杂志,2025,40(8):4035-4040,6.基金项目
国家自然科学基金项目(No.82174130,No.82274262),上海市医院中药制剂产业转化协同创新中心项目,上海市卫生健康委员会科研项目(No.202240053),上海市科学技术委员会项目(No.19401970400),国家中医药管理局高水平中医药重点学科建设项目(No.zyyzdxk-2023065) （No.82174130,No.82274262）
