中华耳科学杂志2025，Vol.23Issue(8)：958-964,7.DOI:10.3969/j.issn.1672-2922.2025.08.009
抗氧化纳米试剂AST@dSe-AFT对顺铂诱导的内耳毛细胞损伤的保护作用观察
Protective Effects of Diselenide Cross-Linked Nano-Loaded Astaxanthin Against Cisplatin-Induced Cochlear Hair Cell Damage
摘要
Abstract
Objective To report protective effects of diselenide cross-linked nano-loaded astaxanthin(AST@dSe-AFT)against cisplatin-induced inner ear hair cell injury.Methods HEI-OC1 hair cells were treated with cisplatin,cisplatin+AST@dSe-AFT or cisplatin+AST.To assess protective effects on hair cells by AST@dSe-AFT,the cell counting kit-8 was used for cell viability,DCFH-DA for reactive oxygen species and Rhodamine123,Mito Tracker Red CMXRos for mitochondrial membrane potentials and immunofluorescence,Western Blot and qPCR for expression of autophagy related proteins.C57BL/6 mice were randomly selected to receive cisplatin,cisplatin+AST or cisplatin+AST@dSe-AFT.Hearing was evaluated by auditory brainstem responses(ABRs)and cochlear basement membrane hair cells by immunofluorescence staining for protection by AST@dSe-AFT against hair cell damage by cisplatin.Results In vitro experiments,treatment with AST@dSe-AFT led to reduced accumulation of reactive oxygen species in cells,with increased mitochondrial membrane potentials and decreased expression of autophagy related proteins and genes induced by cisplatin.In vivo AST@dSe-AFT treatment yielded alleviated cochlear hair cell damage and hearing loss caused by cisplatin.Conclusion Both in vitro and in vivo results confirm that AST@dSe-AFT can effectively protect against cisplatin-induced ototoxicity.关键词
毛细胞/铁蛋白纳米试剂/虾青素/顺铂/氧化应激Key words
hair cell/ferritin nanoparticles/astaxanthin/cisplatin/oxidative stress引用本文复制引用
陈湘,杨思琪,李俊,王子怡,丁恒,于亚峰..抗氧化纳米试剂AST@dSe-AFT对顺铂诱导的内耳毛细胞损伤的保护作用观察[J].中华耳科学杂志,2025,23(8):958-964,7.基金项目
国家自然科学基金课题面上项目(82271162) （82271162）
苏州市科技局项目(SKYD2023106) （SKYD2023106）
苏州大学附属第一医院自然科学基金博习培育项目(BXQN2023037) （BXQN2023037）
