中华耳科学杂志2025，Vol.23Issue(8)：965-971,7.DOI:10.3969/j.issn.1672-2922.2025.08.010
壳聚糖-普鲁士蓝纳米酶对过氧化氢诱导的HEI-OC1细胞氧化应激损伤的影响
Effects of Chitosan-Prussian Blue Nanomases on H2O2 Induced Oxidative Stress Injury of HEI-OC1 Cells
摘要
Abstract
Objective To report effects of chitosan-Prussian blue nanomases(CS-PB)on oxidative stress injury of HEI-OC1 cells induced by H2O2 and related molecular mechanisms.Methods HEI-OC1 cells were cultured and oxidative stress injury established by treatment with H2O2 of various concentration gradients and measurement of intracellular cell counting kit-8 activity.Based on the treatment,these cells were divided into the control,H2O2 treatment,H2O2+CS-PB and H2O2+N-Acetylcysteine groups.Intracellular ROS content was detected by DCFH-DA fluorescence probe technology;intracellular expression levels of Bcl-XL,Bax,TNF-α and IL-1β proteins by Western blot;and content of 4-HNE by immunofluorescence staining and confocal laser microscopy.Results Compared with the control,cell viability and Bcl-XL protein expression in H2O2 treatment cells were decreased(P<0.0001),with increased intracellular reactive oxygen species,4-HNE,TNF-α,IL1β and Bax expression(P<0.0001).Compared with the H2O2 treatment only group,cell viability and Bcl-XL protein expression in H2O2+CS-PB and H2O2+NAC groups were higher(P<0.05),with lower intracellular ROS,4-HNE,TNF-α,IL-1β and Bax expression(P<0.01).Conclusion CS-PB can alleviate cell damage and apoptosis caused by hydrogen peroxide induced oxidative stress in HEI-OC1 cells.关键词
HEI-OC1细胞/纳米酶/活性氧/氧化应激/凋亡Key words
HEI-OC1 cells/nanase/reactive oxygen species/oxidative stress/modulation necrosis引用本文复制引用
郭富余,翟悦怡,杨树玲,魏天洋,张宇轩,李勇,王娜..壳聚糖-普鲁士蓝纳米酶对过氧化氢诱导的HEI-OC1细胞氧化应激损伤的影响[J].中华耳科学杂志,2025,23(8):965-971,7.基金项目
中央引导地方科技发展资金项目(236Z7730G) （236Z7730G）
