种子2025，Vol.44Issue(8)：10-20,29,12.DOI:10.16590/j.cnki.1001-4705.2025.08.010
多刺绿绒蒿bZIP基因家族的鉴定及生物信息学分析
Identification and Bioinformatics Analysis of bZIP Gene Family of Meconopsis horridula
摘要
Abstract
The bZIP transcription factor family plays critical roles in plant growth and development,secondary metabolism regulation,and environmental stress responses.To investigate the functions of the bZIP gene family in the Tibetan medicinal plant Meconopsis horridula,the full-length transcrip-tome data was integrated and members of the bZIP gene family were identified.Subsequent bioinfor-matic analyses were conducted on these genes and their encoded proteins,including physicochemical properties,conserved domains,and phylogenetic relationships.The results demonstrated that 87 bZIP genes were successfully identified,all of which encoded proteins containing the bZIP domain.The amino acid lengths ranged from 107 to 723,molecular weights varied between 12 497.14 Da and 44 951.86 Da,and theoretical isoelectric points ranged from 5.18 to 9.98.Most proteins were predicted to be hydrophilic and unstable,with predominant nuclear localization.Phylogenetic analysis revealed that Meconopsis horridula bZIP proteins were distributed across multiple clades along with Arabi-dopsis thaliana and Oryza sativa bZIP genes,indicating high sequence similarity and conserved evo-lutionary patterns,which suggested potential functional conservation.Tertiary structure predictions showed varying evolutionary modifications among these proteins,incorporating spatial conformations such as α-helices,β-turns,random coils,and extended strands.关键词
多刺绿绒蒿/bZIP基因家族/生物信息学/全长转录组Key words
Meconopsis horridula/bZIP gene family/bioinformatics/full-length transcriptome分类
农业科技引用本文复制引用
李真,马兰民,张文惠平,东主南加,孙胜男..多刺绿绒蒿bZIP基因家族的鉴定及生物信息学分析[J].种子,2025,44(8):10-20,29,12.基金项目
国家自然科学基金地区项目(32160233) （32160233）
