种子2025，Vol.44Issue(8)：21-29,9.DOI:10.16590/j.cnki.1001-4705.2025.08.021
蓖麻Caleosin基因家族鉴定及碱胁迫下表达分析
Identification of the Caleosin Gene Family in Ricinus communis L.and Analysis of Expression under Alkali Stress
摘要
Abstract
Members of the castor Caleosin gene family were screened out using Ricinus communis pro-tein sequence files.Various properties of the gene family members and encoded proteins were predic-ted and analyzed by bioinformatics methods.The expression patterns of caleosin gene family members at different base concentrations of two Ricinus communis varieties with significant differences in alkali tolerance during the germination period were determined by real-time fluorescence quantitative reverse transcription PCR(RT-qPCR).Two members,RcCLO1 and RcCLO2,were identified in the Ricinus communis protein sequence files.They belonged to the H-Caleosin subfamily and the L-Caleosin sub-family respectively,and evolved from each of the two subfamilies.The evolution of RcCLO1 and RcCLO2 was highly conserved,and both were as the key functions and conserved motifs of caleosin.Both RcCLO1 and RcCLO2 could respond to alkali stress,and RcCLO1 could be the main alkali-re-sistant gene in the Ricinus comrnunis caleosin gene family.关键词
蓖麻/Caleosin基因家族/生物信息学/碱胁迫Key words
Ricinus communis L./Caleosin gene family/bioinformatics/alkali stress分类
农业科技引用本文复制引用
杜亚彤,徐元静,李月明,韩沛霖,王竞红,蔺吉祥..蓖麻Caleosin基因家族鉴定及碱胁迫下表达分析[J].种子,2025,44(8):21-29,9.基金项目
黑龙江省自然科学基金项目(LH2024C036) （LH2024C036）
