种子2025，Vol.44Issue(8)：54-64,73,12.DOI:10.16590/j.cnki.1001-4705.2025.08.054
云南铁壳麦重组自交系群体穗发芽抗性鉴定
Identification for Spike Sprouting Resistance of the Recombinant Inbred Population of Yunnan Hull Wheat
摘要
Abstract
Wheat spike sprouting can seriously affect the quality and grade of wheat(Triticum aestivum L.)in production.Screening resistant germplasm and using functional molecular markers can funda-mentally reduce or avoid the harm of wheat spike germination.Taking 298 high-generation recombi-nant inbred lines,namely Lincang Hull Wheat and highly sensitive Yunmai53,which were identified as highly resistant to spike germination in the early stage,as materials,the spike germination resist-ance was identified and evaluated by two methods:whole spike germination and grain germination.Molecular markers were used to detect the main spike germination resistation-related genes(TaMKK3-A,TaSdr-2B,Tamyb10-D1,TaMFT-A2,GS34-7B,TaMFT-A1,TaDFR-3B,Tamyb10-B1,TaSdr-2A,and Tamyb10-A1).the effects of different resistance genes in combination with their phenotypes were analysed.The results showed that the average whole spike germination rate(SGR)of 298 materials was 86.79％,with a variation range of 0.13％to 100.00％.The average germination index(GI)was 56.85％,with a variation range of 0.43％to 100.00％.Among them,the whole spike germination rate values of 7 materials and the grain germination rate values of 32 materials reached the high resistance level.The whole spike germination rate values of 7 materials and the grain germi-nation rate values of 27 materials reached the resistance level.The relative germination index values of the whole spike of 2 materials and the relative germination index values of 14 grains reached the resistance level compared with Yunmai53.When the relative germination index was used as the classi-fication standard,the relative germination index values of the whole spike of 2 materials and the rela-tive germination index values of 14 grains reached the high resistance grade compared with Yunmai53,and could be utilized as germplasm resources resistant to spike germination.Among the 10 genes related to spike germination resistance,TaDFR-3Bb had the highest distribution frequency,reaching 68.71％.The other genes were TaMFT-A1b(63.85％),TaMFT-A2b(61.96％),Tamyb10-B1b(57.50％),and Tamyb10-B1a(43.33％)in sequence,TaMFT-A2a(38.65％),TaMFTA1a(37.09％),TaDFR-3Ba(32.52％),GS34-7B(0).Combined with phenotypic analysis,it was found that TaMFT-A2,TaMFT-A1,TaDFR-3Ba,and Tamyb10-B1 were not correlated with the pheno-type.TaMKK3-A,TaSdr-2B,Tamyb10-D1,TaSdr-2A,and Tamyb10-A1 were significantly associ-ated with phenotypes.The absence of bands in GS34-7B indicated that the material did not contain this resistance gene.Therefore,molecular markers such as TaMKK3-A,TaSdr-2B,Tamyb10-D1,TaSdr-2A,and Tamyb10-A1 could be utilized.关键词
小麦穗发芽抗性/整穗发芽率/籽粒发芽率Key words
sprouting resistance of wheat spike/whole spike germination rate/grain germination rate分类
农业科技引用本文复制引用
周国雁,程莎,隆文杰,刘圆圆,胡海瑞,孙梓菁,蔡青,陈丹..云南铁壳麦重组自交系群体穗发芽抗性鉴定[J].种子,2025,44(8):54-64,73,12.基金项目
云南省基础研究计划项目"云南铁壳麦穗发芽抗性机制研究"(202301AT070010) （202301AT070010）
云南省种子种业联合实验室项目"作物种质资源数据库与共享平台建设"(202205AR0000-10) （202205AR0000-10）
农业农村部"小麦种质资源抗穗发芽性状精准鉴定"(19240576) （19240576）
云南省农业基础研究联合专项"云南铁壳麦参考基因组构建与优异基因挖掘"(202501BD070001-012) （202501BD070001-012）
科技部、财政部"国家科技资源共享服务平台—国家作物种质资源库"云南农作物种质资源保存利用与共享服务"(NCGRC2025-030) （NCGRC2025-030）
2024年省级农业发展专项资金"云南省省级农作物种质资源库建设" （）
