种子2025，Vol.44Issue(8)：85-93,9.DOI:10.16590/j.cnki.1001-4705.2025.08.085
基于广泛靶向代谢组学的'怀枝'和'黑叶'互接差异亲和表现及代谢物差异分析
Analysis of Differential Affinity and Metabolite Differences between 'Huaizhi'and 'Heiye' Litchis Based on Broadly Targeted Metabolomics
摘要
Abstract
In order to preliminarily understand the metabolic substances that affect the grafting affinity of Litchi chinensis,ultra-performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)technology was used to conduct extensive targeted metabolomics studies on the grafting strong affinity combination'Huanzhi(rootstock)-Heiye(scion)'and weak affinity combination'Heiye(root-stock)-Huaizhi(scion)'.The results showed that a total of 1 166 metabolites were identified in the samples.Among the two grafting combinations,'Huaizhi(rootstock)-Heiye(scion)'and'Heiye(root-stock)-Huaizhi(scion)',41 differential metabolites were screened out.The differential metabolites mainly included flavonoids,phenolic acids,amino acids and their derivatives,etc.The criteria for jud-ging the strength of grafting affinity of Litchi chinensis after grafting were preliminarily screened out substance of nature.KEGG enrichment analysis of differential metabolites revealed that the main met-abolic pathways significantly enriched by differential metabolites were flavonoid biosynthesis,folic acid biosynthesis,inositol phosphate metabolism,glycerol phosphate lipid metabolism,flavonoid bio-synthesis,starch and sucrose metabolism,etc.关键词
荔枝/嫁接亲和性/代谢组学/差异代谢物Key words
Litchi chinensis/graft compatibility/metabolomics/differential metabolites分类
农业科技引用本文复制引用
侯延杰,王明元,李鸿莉,房晨,赵博翔,李冬波,尤婧祎,邱宏业,李晓龙,徐宁,秦献泉..基于广泛靶向代谢组学的'怀枝'和'黑叶'互接差异亲和表现及代谢物差异分析[J].种子,2025,44(8):85-93,9.基金项目
基于形态学位置效应的荔枝嫁接亲和性机制的多组学研究(2022GXNSFBA035559) （2022GXNSFBA035559）
国家荔枝龙眼产业技术体系良种繁育与生产技术配套岗位项目(CARS-32-02) （CARS-32-02）
广西荔枝龙眼创新团队-育种与栽培岗位(nycytxgxcxtd-2023-12-02) （nycytxgxcxtd-2023-12-02）
广西荔枝新品种选育及示范应用(桂科AA23023007 （）
桂科AA23023007-3) （）
广西荔枝保花保果与抗逆提质栽培技术研发与应用(2023YFD2300805-6) （2023YFD2300805-6）
荔枝龙眼种质资源研究与品种选育(桂农科2021YT043) （桂农科2021YT043）
