种子2025，Vol.44Issue(9)：1-10,10.DOI:10.16590/j.cnki.1001-4705.2025.09.001
JAZ2.2对木薯细菌性枯萎病抗性调控机制探究
Exploration of the Regulatory Mechanism of Resistance to Bacterial Fusarium Wilt in Manihot esculenta by JAZ2.2
摘要
Abstract
Jasmonate ZIM domain(JAZ)protein,as a key inhibitory factor in the jasmonic acid signal transduction pathway,plays an important role in the resistance of plants to pathogens.By taking ad-vantage of the negative regulatory effect of MeJAZ2.2 on Manihot esculenta resistance to bacterial fu-sarium wilt,the molecular mechanism was further analyzed.ACO3(1-AMINOCYCLOPROPANE-1-CARBOXYLATE OXIDASE 3-RELATED)was screened as a candidate interacting protein through yeast two-hybrid experiments.Subsequently,the phenotypes of the MeACO3 gene-silenced strains and the co-silenced strains of MeJAZ2.2 and MeACO3 after inoculation with bacterial fusarium wilt pathogenic bacteria were analyzed.The research results showed that the yeast two-hybrid experiment,Pulldown experiment and bilayer fluorescence complementarity experiment confirmed the interaction between MeJAZ2.2 and MeACO3 in vivo and in vitro.qRT-PCR analysis revealed that the expression level of MeACO3 in Manihot esculenta plants increased by 9 times 6 hours after Xam inoculation com-pared with the control group,suggesting that MeACO3 might be involved in the immune response of Manihot esculenta to bacterial fusarium wilt.MeACO3 subcellular sites were located in the nucleus and plas-ma membrane.Compared with the control line,the lesion area on the Manihot esculenta leaves of the MeA-CO3 silenced strain was larger after Xam inoculation.The lesion area of the co-silenced MeJAZ2.2-MeACO3 strain was smaller than that of the MeACO3 silenced strain,indicating that MeJAZ2.2 and MeACO3 could antagonize and regulate the resistance of Manihot esculenta to bacterial wilt disease.关键词
木薯/细菌性枯萎病/MeJAZ2.2/蛋白互作Key words
Manihot esculenta/bacterial fusarium wilt/MeJAZ2.2/protein interaction分类
农业科技引用本文复制引用
闫文雷,杨澜,曾鸿秋..JAZ2.2对木薯细菌性枯萎病抗性调控机制探究[J].种子,2025,44(9):1-10,10.基金项目
国家自然科学基金(32401763) （32401763）
