种子2025,Vol.44Issue(9):81-87,7.DOI:10.16590/j.cnki.1001-4705.2025.09.081
甘蓝型油菜籽粒密度QTL位点cqSD-A9的SSR标记开发及应用
The Development and Application of SSR Markers for the QTL Locus cqSD-A9 Related to Seed Density in Brassica napus
摘要
Abstract
Seed density per silique is one of the important traits affecting Brassica napus yield and plays a significant role in increasing rapeseed yield.In previous studies,it was found that the grain density site cqSD-A9 could be stably detected in multiple environments.To construct a new BC1 popu-lation using the No.3879 and No.3383 strains selected from the DH lines and the spring Brassica na-pus'QU'as parents,SSR molecular markers were developed using the Darmor-bzh'reference genome sequence.the BC1 population was detected and preliminarily located using polymorphic markers.The results indicated that six SSR markers were closely linked to the QTL of Seed density per silique on chromosome A9,reducing the interval from 23.82-29.19 Mb to 26.58-28.49 Mb.Meanwhile,a finely positioned population was constructed using marker-assisted selection.关键词
甘蓝型油菜/籽粒密度/cqSD-A9位点/SSR分子标记Key words
Brassica napus L./seed density/cqSD-A9 locus/SSR molecular markers分类
农业科技引用本文复制引用
王浩君,谢立人,李开祥..甘蓝型油菜籽粒密度QTL位点cqSD-A9的SSR标记开发及应用[J].种子,2025,44(9):81-87,7.基金项目
青海省春油菜遗传改良重点实验室(2024) (2024)
国家油菜产业技术体系项目(CARS-12) (CARS-12)