浙江农业学报2025，Vol.37Issue(9)：1825-1839,15.DOI:10.3969/j.issn.1004-1524.20240599
小麦SWEET和SUT家族基因鉴定与表达分析
Identification and expression analysis of members of the SWEET and SUT families in wheat(Triticum aestivum L.)
摘要
Abstract
SWEETs(sugars will eventually be exported transporter)and SUTs(sucrose transporters)are two sugar transporters in plants that play important roles in carbohydrate transport.In order to analyze the evolutionary charac-teristics,functions and responses of TaSWEET and TaSUT genes in the wheat genome and their response to exoge-nous sugars,this study conducted genome-wide identification of wheat TaSWEET and TaSUT,and analyzed their phylogenetic relationships,gene structure,promoter sequence elements,and expression characteristics.101 Ta-SWEET genes and 16 TaSUT genes had been identified in wheat genome,both of which could be divided into four subfamilies.The genetic structure exhibited differences in the number of exons and introns among the TaSWEET fam-ily and TaSUT family members,ranging from 4 to 18.Conservative motif analysis displayed that the TaSWEET family and TaSUT family members had 20 motifs,the motif structures within each subfamily were similar.Chromosome dis-tribution pattern and homology analysis indicated that TaSWEET family and TaSUT family members were evenly dis-tributed in the three subgenomes,and the segmental and tandem repeats of the whole genome promoted the amplifica-tion of TaSWEET genes.Subcellular localization prediction proved that most of the TaSWEET and all TaSUT mem-bers were located in the plasma membrane.TaSWEET contained 4-7 transmembrane helical structures,while TaSUT contained 8-12.The analysis of promoter elements forecasted that the TaSWEET and TaSUT families were rich in growth and development,hormone response and abiotic stress cis-acting elements.Wheat gene expression pat-tern analysis demonstrated that most of the TaSWEET and TaSUT family members expressed at low levels in wheat leaves,while 28,25,33,39 and 21 members expressed at high levels in leaf,shoot,root,spike and grain,respec-tively.Through qRT-PCR detection,it was found that the expression levels of TaSWEET and TaSUT family genes were all down-regulated in roots after exogenous sugar treatment,while 3 genes were up-regulated in leaves under fructose treatment,and one gene was up-regulated under all three suger treatments.The above results provide a theo-retical basis for further studying the regulation of growth and development of TaSWEET and TaSUT genes in wheat and their response to exogenous sugar treatment.关键词
小麦/TaSWEET家族/TaSUT家族/糖转运蛋白/基因表达Key words
wheat/TaSWEET family/TaSUT family/sugar transporter/gene expression分类
农业科技引用本文复制引用
张均,马超,张博,胡碧博,刘京亮,张晓宇,李春阳,熊盛婷,郭彬彬,王秀存..小麦SWEET和SUT家族基因鉴定与表达分析[J].浙江农业学报,2025,37(9):1825-1839,15.基金项目
国家自然科学基金(32372227) （32372227）
河南省高等学校青年骨干教师培养计划(2021GGJS050) （2021GGJS050）
河南省科技研发计划联合基金项目(232103810021) （232103810021）
河南科技大学大学生创新创业训练计划项目(2024425,2024427) （2024425,2024427）
