浙江农业学报2025，Vol.37Issue(9)：1860-1871,12.DOI:10.3969/j.issn.1004-1524.20240563
五十四份茶树种质的特征性代谢产物含量与耐寒性
Characteristic metabolite content and cold resistance of 54 tea germplasms
摘要
Abstract
To breed new tea plant cultivars with cold resistance and high quality,and clarify the correlation between characteristic metabolite content and cold tolerance in tea plants,the freezing injury index of 54 tea germplasms was obtained through field investigation.High-performance liquid chromatography(HPLC)was used to detect 27 bio-chemical components.Biochemical components which were closely related to cold resistance were identified through significance analysis of difference,correlation analysis,and path analysis.The results showed that among the 54 germplasms,16 exhibited highly cold resistance and 7 showed highly cold sensitivity.Caffeine,epigallocatechin gal-late(EGCG),and theanine were the predominant alkaloid,catechin,and amino acid components,respectively,with average contents of 32.96,54.89,and 24.17 mg·g-1.The average coefficient of variation and genetic diversity in-dex of amino acids were higher than those of alkaloids and catechins.The EGCG content in highly cold resistant germplasms was significantly(p＜0.01)higher than that in highly cold sensitive germplasms.Conversely,the con-tents of nine amino acids,including theanine,serine,and threonine,were significantly(p＜0.05 or p＜0.01)low-er in highly cold resistant germplasms.Correlation analysis revealed a highly significant(p＜0.01)negative correla-tion between EGCG and freezing injury index,while eleven amino acids(including alanine,serine,leucine and so on)showed significant(p＜0.05 or p＜0.01)positive correlations with freezing injury index.Path analysis showed that serine and glutamic acid are the biochemical components with the greatest impact on the tea plant freezing injury index,with their contribution rates to the freezing injury index being 0.53 and 0.50,respectively.They might be key biochemical components affecting cold resistance in tea plants.Based on the content of biochemical components and freezing injury index,six tea germplasms with both strong cold resistance and high theanine content were select-ed,which are suitable for high-altitude mountain tea regions in Guizhou Province.Among them,GT-GY-05 and GT-AS-81 exhibited strong cold resistance and high theanine content(≥ 30.0 mg·g-1),making them suitable for breeding new cold resistant and high quality tea varieties.关键词
茶树/冻害指数/咖啡碱/表没食子儿茶素没食子酸酯(EGCG)/茶氨酸/氨基酸Key words
tea plant/freezing injury index/caffeine/epigallocatechin gallate(EGCG)/L-theanine/amino acid分类
农业科技引用本文复制引用
杨春,郭燕,梁思慧,汪安然,陈娟,李燕,林开勤,密孝增,乔大河,陈正武..五十四份茶树种质的特征性代谢产物含量与耐寒性[J].浙江农业学报,2025,37(9):1860-1871,12.基金项目
贵州省科技计划项目(黔科合支撑[2021]一般189) （黔科合支撑[2021]一般189）
贵州省农业科学院喀斯特山区作物基因资源与种业创新重点实验室项目(黔农科院种质资源[2023]03号) （黔农科院种质资源[2023]03号）
喀斯特山区特色作物生物育种创新能力建设(黔科合服企[2024]003-1) （黔科合服企[2024]003-1）
