浙江农业学报2025，Vol.37Issue(9)：1914-1923,10.DOI:10.3969/j.issn.1004-1524.20240920
瓠瓜EMS诱变突变体筛选与表型分析
Screening and phenotypic analysis of EMS-induced mutants in Lagenaria siceraria
摘要
Abstract
To investigate suitable ethyl methanesulfonate(EMS)mutagenesis conditions and screen for mutants in bottle gourd(Lagenaria siceraria),seeds of the local variety Hangzhou Long Gourd were treated with different vol-ume fractions of EMS and soaking time to determine the optimal mutagenesis parameters.Subsequently,these opti-mal conditions were applied to construct an EMS-induced mutant library.The results showed that the optimal EMS mutagenesis condition for bottle gourd was soaking seeds in 1.2％EMS solution for 12 h,under which the seed ger-mination rate was 49.4％.Using this mutagenesis condition,an EMS mutant library containing 667 M,generation plants was established.From this library,14 mutants exhibiting various phenotypes were identified,including yello-wing leaf,wrinkled leaf,albino lethal plant,albino leaf,disease spot leaf,dwarf plant,short fruit and albino peel.The mutation rate was 2.10％.Phenotypic characterization and ultrastructural observation revealed that one yellowing leaf mutant,X-67,possessed lower photosynthetic pigment content and incomplete chloroplast development,poten-tially explaining its juvenile leaf yellowing phenotype.This study identified the optimal EMS mutagenesis conditions for bottle gourd and obtained a collection of EMS-induced mutants,providing valuable material resources for function-al genomics research in bottle gourd.关键词
瓠瓜/甲基磺酸乙酯/突变体/叶色黄化Key words
bottle gourd/ethyl methane sulfonate/mutant/yellowing leaf分类
农业科技引用本文复制引用
郑程,汪颖,王尖,郭笑,汪宝根,吴新义,祝彪,李国景,吴晓花..瓠瓜EMS诱变突变体筛选与表型分析[J].浙江农业学报,2025,37(9):1914-1923,10.基金项目
浙江省农业(蔬菜)新品种选育重大科技专项(2021C02065-2-1) （蔬菜）
