针灸推拿医学（英文版）2025，Vol.23Issue(5)：377-384,8.DOI:10.1007/s11726-025-1525-x
应用10.6μm激光灸足三里和关元调控荷瘤小鼠肿瘤相关免疫
Modulation of tumor-related immunity by 10.6 μm laser moxibustion at Zusanli(ST36)and Guanyuan(CV4)in tumor-bearing mice
摘要
Abstract
Objective:To evaluate the effects of laser moxibustion(LM)on tumor-related immunity in tumor-bearing mice. Methods:Thirty male C57BL/6 mice were randomized into a control group,a model group,and an LM group,with 10 mice in each group.Mice in the control group did not receive any intervention,and mice in the model group and LM group were injected into the right armpit with 1×107 cells/mL Lewis lung carcinoma cells to induce tumor models.From the first day after the injection,the LM group mice were irradiated with LM at Guanyuan(CV4)and bilateral Zusanli(ST36),5 min each point for a total of 15 min,once daily for 15 consecutive days.From the day of visible tumor formation(i.e.,day 8),mice in the model and LM groups were measured every other day with a vernier caliper to calculate the tumor volume.On day 16 after injection,the serum,lung,spleen,and tumors of the mice were harvested.Hematoxylin-eosin staining was used to observe tumor pulmonary metastasis.Changes in the serum levels of programmed death 1(PD-1)and programmed death-ligand 1(PD-L1)were analyzed by enzyme-linked immunosorbent assay;the abundance of PD-1 and PD-L1 proteins in tumors was determined by Western blotting;and the proportions of lymphocyte subsets and myeloid-derived suppressor cells(MDSCs)in tumors and spleen were determined by flow cytometry. Results:Compared to the model group,the tumor volume of mice in the LM group decreased significantly on days 8 and 10(P<0.05);the lung tissues of the LM group showed no apparent tumor atypia;the proportion of monocytic MDSCs(M-MDSCs)in the tumors of LM group mice decreased(P<0.05);the PD-1 and PD-L1 levels in the serum of mice in the LM group declined with statistically significant variation in the reduction of PD-L1(P<0.01);PD-1 and PD-L1 protein expression in the tumor tissues of mice in the LM group reduced significantly(P<0.01). Conclusion:LM intervention can reduce the expression of PD-1 and PD-L1 in the serum of tumor-bearing mice,decrease the expression of PD-1 and PD-L1 proteins and the proportion of M-MDSCs in tumor tissues.These effects may be one of the mechanisms by which LM slows the early-stage growth of Lewis lung carcinoma tumor in mice.关键词
灸法/激光治疗/肿瘤免疫/程序性死亡受体1/程序性死亡配体1/髓源性抑制细胞/小鼠Key words
Moxibustion Therapy/Laser Therapy/Tumor Immunity/Programmed Death 1/Programmed Death-ligand 1/Myeloid-derived Suppressor Cells/Mice分类
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秦梦,吴晓波,姜玉姣,魏建子..应用10.6μm激光灸足三里和关元调控荷瘤小鼠肿瘤相关免疫[J].针灸推拿医学（英文版）,2025,23(5):377-384,8.基金项目
This work was supported by the Projects of National Natural Science Foundation of China(国家自然科学基金项目,No.81804194,No.82274640) （国家自然科学基金项目,No.81804194,No.82274640）
General Project of Shanghai Municipal Natural Science Foundation(上海市自然科学基金面上项目,No.21ZR1463300). （上海市自然科学基金面上项目,No.21ZR1463300）
