中国现代医生2025，Vol.63Issue(27)：46-52,7.DOI:10.3969/j.issn.1673-9701.2025.27.010
基于网络药理学和分子对接探讨二陈平胃散治疗伴胰岛素抵抗肥胖症的机制
Discussion on the mechanism of Erchen Pingwei formula treating obesity with insulin resistance based on network pharmacology and molecular docking
摘要
Abstract
Objective To investigate the potential mechanism of Erchen Pingwei formula in treating obesity with insulin resistance(IR)by using network pharmacology and molecular docking technology.Methods The main active ingredients and their potential target proteins were obtained from Traditional Chinese Medicine System Pharmacology database and analysis platform and Swiss Target Prediction databases,therapeutic target database,online Mendelian inheritance in man database and genecards databases were used to screen the disease targets related to IR and obesity,and Venny mapping tool was used to screen the intersection of herbal formula and disease targets,and Cytoscape 3.10.0 software was used to construct protein-protein interaction(PPI)network diagrams.Gene Ontology(GO)enrichment,Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway analysis on the intersection targets were conducted by using DAVID database,molecular docking and visualization analysis were carried out by Autodock and Pymol.Results 136 active ingredients and 236 active targets of Erchen Pingwei formula were retrieved,with 2527 IR active targets,1598 obesity active targets,and 91 drug-disease intersecting targets.GO and KEGG enrichment analyses showed that quercetin,kaempferol,and naringenin were the main active ingredients of Erchen Pingwei formula,through the regulation of protein kinase B,interleukin-6,tumor protein p53,peroxisome proliferator activated receptor,estrogen receptor α and other core targets,affecting lipid and atherosclerosis,insulin resistance,advanced glycation end product-receptor signaling pathway,and playing a therapeutic role in obesity.Conclusion Erchen Pingwei formula treats obesity with IR via a multi-component,multi-target,and multi-pathway mechanism.关键词
二陈平胃散/胰岛素抵抗/肥胖症/网络药理学Key words
Erchen Pingwei formula/Insulin resistance/Obesity/Network pharmacology分类
医药卫生引用本文复制引用
王凯,翁思颖..基于网络药理学和分子对接探讨二陈平胃散治疗伴胰岛素抵抗肥胖症的机制[J].中国现代医生,2025,63(27):46-52,7.基金项目
国家自然科学基金资助项目(82004325 （）
82474424) （）
宁波市"科创甬江2035"关键技术项目(2024Z282) （2024Z282）
