中医药学报2025，Vol.53Issue(10)：29-34,6.DOI:10.19664/j.cnki.1002-2392.250205
百部抑制巨噬细胞趋化作用治疗肺动脉高压的分子机制研究
Molecular Mechanism of Stemonae Radix in Inhibiting Macrophage Chemotaxis for Treating Pulmonary Hypertension
摘要
Abstract
Objective:To investigate the effects and mechanisms of Stemonae Radix(SR)on macrophage recruitment to lung tissue and inflammatory factor expression in mice with pulmonary hypertension(PH).Methods:Human pulmonary artery endothelial cells(HPAECs)were divided into a control group and a model group.ELISA was used to detect the effect of hypoxia on HPAEC secretion of chemokine ligand 9(CXCL9)and chemokine ligand 10(CXCL10).THP-1 cells were divided into a control group,model group,low-dose SR group,and high-dose SR group.Flow cytometry was used to assess the effect of SR on chemokine receptor 3(CXCR3)expression in hypoxic THP-1 cells,and a transwell assay was conducted to verify SR's inhibitory effect on THP-1 cell chemotaxis under hypoxia.Mice were divided into a control group,model group,and SR group.Right ventricular systolic pressure(RVSP)and right ventricular hypertrophy index(RVHI)were measured to evaluate SR's therapeutic effect on PH mice.Lung tissue immunofluorescence staining was performed to observe macrophage aggregation,and ELISA was used to detect IL-1β,IL-6,and TNF-α levels in lung tissue.Results:ELISA results showed that CXCL9 and CXCL10 levels secreted by hypoxic HPAECs were significantly higher than those in the control group(P<0.01).Flow cytometry revealed that CXCR3 expression in hypoxic macrophages was significantly increased(P<0.01),which was markedly reduced after treatment with low-and high-dose SR(P<0.01).Both low-and high-dose SR inhibited macrophage migration in the model group(P<0.05,P<0.01).SR alleviated RVSP and RVHI in PH mice,and immunofluorescence showed reduced macrophage aggregation in lung tissue,with statistically significant differences(P<0.01).After SR treatment,IL-1β,IL-6,and TNF-α levels in lung tissue were significantly decreased(P<0.05,P<0.01).Conclusion:SR reduces CXCR3 expression on macrophages,inhibits their chemotaxis toward CXCL9 and CXCL10,decreases migration to lung tissue,and lowers inflammatory factor levels,thereby ameliorating pulmonary hypertension symptoms.关键词
百部/肺动脉高压/巨噬细胞/CXCR3Key words
Stemonae Radix/Pulmonary hypertension/Macrophage/CXCR3分类
医药卫生引用本文复制引用
倪兴彬,常亚娟,于佳远,肖爽,王兴业,周良钰..百部抑制巨噬细胞趋化作用治疗肺动脉高压的分子机制研究[J].中医药学报,2025,53(10):29-34,6.基金项目
黑龙江省卫健委科研课题(20211313010353) （20211313010353）
黑龙江省中医药科研项目(ZHY2025-024) （ZHY2025-024）
