中华中医药学刊2025，Vol.43Issue(10)：28-32,后插2-后插4,8.DOI:10.13193/j.issn.1673-7717.2025.10.005
锁阳黄酮改善SAMP8小鼠认知功能障碍的机制研究
Mechanism of Cynomorium songaricum flavonoids in Improving Cognitive Dysfunction in SAMP8
摘要
Abstract
Objective To investigate the effect and mechanism of Cynomorium songaricum flavonoids(CSF)on cognitive dys-function in senescence-accelerated mice prone 8(SAMP8).Methods Fifty 7-month-old male SAMP8 were randomly divided into control group,low,intermediate and high dose groups of CSF[25,50,100 mg/(kg·d)],and donepezil group.The low,in-termediate and high dose groups were given a corresponding dose of CSF to SAMP8 mice by continuous gavage for 20 days,while the donepezil group was given donepezil[0.71 mg/(kg·d)]to SAMP8 mice by gavage for 20 days.The spatial learning and memory performance was evaluate by the Morris water maze experiment after administration,and the morphology and count of hip-pocampal pyramidal cells was observed by Nissl staining.The apoptosis-related protein expression levels and the levels of nu-clear factor E2 related factor 2(Nrf2),Kelch epichlorohydrin associated protein 1(Keap1)and quinone oxidoreductase-1(NQO1)in the hippocampus of each group were detected by Western blotting.The serum levels of malondialdehyde(MDA)con-tent as well as glutathione peroxidase(GSH-Px)and superoxide dismutase(SOD)activities were measured by ELISA.Results CSF significantly reduced the escape latency and path length,while increased the number of crossing platforms and the percentage of target quadrant time(P＜0.05).CSF improved the morphology and quantity of pyramidal cells in hippocampal CA1 region.Western blot results showed that compared with the control group,the protein expression levels of Nrf2,Keap1 and NQO1 in the hippocampus of the intermediate dose group,high dose group and donepezil group all significantly increased(P＜0.05),the ratio of Bcl-2/Bax protein expression in the hippocampus of all administration groups of CSF and donepezil group in-creased substantially(P＜0.05),and the expressions of Caspase-3 protein in the hippocampus of the intermediate dose group,high dose group and donepezil group all decreased significantly(P＜0.05).Compared with the control group,CSF significantly increased the serum activities of SOD and GSH-Px in SAMP8 mice(P＜0.05),while remarkedly decreased the content of MDA(P＜0.05).Conclusion CSF can significantly improve spatial learning and memory impairment in Alzheimer's disease,and its mechanism may be related to inhibiting oxidative stress and cell apoptosis.关键词
锁阳黄酮/阿尔茨海默病/空间学习记忆/凋亡/氧化应激Key words
Cynomorium songaricum flavonoids/Alzheimer disease/spatial learning and memory/apoptosis/oxidative stress分类
医药卫生引用本文复制引用
胡哲,宋嵬,王慧茹,张剑平,秦明凯,向桂珍,张士滨,闫旭升,贾建新..锁阳黄酮改善SAMP8小鼠认知功能障碍的机制研究[J].中华中医药学刊,2025,43(10):28-32,后插2-后插4,8.基金项目
国家自然科学基金项目(82360264) （82360264）
内蒙古自然科学基金项目(2020MS08079,2024MS08051,2025MS08015) （2020MS08079,2024MS08051,2025MS08015）
包头医学院青苗计划项目(BYJJ-ZRQM202208,BYJJ-QM-2018003,BYJJ-ZRQM202310) （BYJJ-ZRQM202208,BYJJ-QM-2018003,BYJJ-ZRQM202310）
包头医学院花蕾计划项目(HLJH202507,HLJH202525) （HLJH202507,HLJH202525）
