安徽农业科学2025，Vol.53Issue(20)：1-6,6.DOI:10.3969/j.issn.0517-6611.2025.20.001
玉米跨膜蛋白ZmLAZ1家族的信号肽鉴定与分泌功能分析
Identification and Secretory Function Analysis of Signal Peptides of the ZmLAZ1 Family of Maize Transmembrane Proteins
摘要
Abstract
[Objective]To investigate the biological function and signal peptide characteristics of the maize Lazarus 1(LAZ1)proteins.[Meth-od]The transmembrane structure and signal peptides of nine members of the ZmLAZ1 family were analyzed using bioinformatic method.The signal peptide secretion function was verified using a pSUC2 vector in combination with the yeast strain YTK12,which has a sucrose invertase secretion defect.[Result]All members of the ZmLAZ1 family contain a high-probability transmembrane helix at the N-terminus.Among them,ZmLAZ1-2,ZmLAZ1-3,ZmLAZ1-4,ZmLAZ1-5,ZmLAZ1-7,and ZmLAZ1-9 were predicted to have signal peptides.Yeast secretion experiments further confirmed that the ZmLAZ1-7 signal peptide possesses secretion activity and the ZmLAZ1-7 signal peptide fusion protein were capable of secreting invertase,enabling the breakdown of raffinose into monosaccharides.[Conclusion]This study provides theoretical support for further elucidating the biological functions of ZmLAZ1 family proteins and their fictional mechanisms in maize,and provides a reference for the study of gene functions and breeding applications in maize.关键词
玉米/跨膜蛋白/ZmLAZ1/信号肽/鉴定/分泌功能Key words
Maize/Transmembrane protein/,ZmLAZ1/Signal peptide/Identification/Secretion function分类
农业科技引用本文复制引用
刘柄良,刘名荣,陶阳,王新惠..玉米跨膜蛋白ZmLAZ1家族的信号肽鉴定与分泌功能分析[J].安徽农业科学,2025,53(20):1-6,6.基金项目
国家自然科学基金项目(32300318) （32300318）
四川省自然科学基金项目(2024NSFSC1202,2024NSFSC1324). （2024NSFSC1202,2024NSFSC1324）
