安徽农业科学2025，Vol.53Issue(20)：7-14,8.DOI:10.3969/j.issn.0517-6611.2025.20.002
过表达CGLD27 LIKE影响拟南芥类胡萝卜素的合成
Overexpression of CGLD27 LIKE Affects the Synthesis of Carotenoids in Arabidopsis thaliana
摘要
Abstract
[Objective]To investigate the evolutionary origin,subcellular localization,and functional role of the CGLD27 LIKE(AT5G11840)protein in carotenoid biosynthesis in Arabidopsis thaliana,as well as its influence on plant growth and photoprotection.[Method]The evolution-ary origin and phylogenetic distribution of CGLD27 LIKE were analyzed using bioinformatics tools.Transgenic plants overexpressing CGLD27 LIKE were generated through molecular biology techniques.Expression levels of genes involved in the carotenoid biosynthesis pathway and the accumulation of photosynthetic pigments in the transgenic lines were quantitatively assessed.[Result]CGLD27 LIKE is of cyanobacterial origin and is highly conserved across photosynthetic organisms.Subcellular localization analysis revealed its chloroplast localization.Overexpression of CGLD27 LIKE resulted in phenotypic alterations,including dwarfism,chlorosis,and reduced fertility.Photosynthetic pigment content was signifi-cantly decreased.The albino phenotype exhibited light-dependent characteristics:it was exacerbated under short-day conditions but partially al-leviated under continuous light exposure.qRT-PCR analysis demonstrated down-regulation of key rate-limiting enzyme-encoding genes such as PSY and its downstream gene LCYb,whereas PDS and ZDS were significantly up-regulated,indicating the presence of a feedback regulatory mechanism in carotenoid biosynthesis.[Conclusion]As an evolutionarily conserved chloroplast-localized protein,CGLD27 LIKE may act as a negative regulator of carotenoid biosynthesis by modulating PSY expression.Its overexpression disrupts photoprotective mechanisms and impairs plant development,thereby providing a novel genetic target for improving crop stress tolerance and nutritional quality.关键词
拟南芥/类胡萝卜素/CGLD27 LIKE/合成Key words
Arabidopsis thaliana/Carotenoid/CGLD27 LIKE/Synthesis分类
农业科技引用本文复制引用
胡丽娟,梁岗..过表达CGLD27 LIKE影响拟南芥类胡萝卜素的合成[J].安徽农业科学,2025,53(20):7-14,8.基金项目
云南省高层次科技人才及创新团队选拔专项(202305AS350010). （202305AS350010）
