北京林业大学学报2025，Vol.47Issue(10)：84-95,12.DOI:10.12171/j.1000-1522.20250179
油松PtDof基因家族鉴定与PtDof1基因响应低温胁迫的功能分析
Identification of PtDof gene family and functional analysis of PtDof1 gene in response to low temperature stress in Pinus tabuliformis
摘要
Abstract
[Objective]This paper identifies and explores the role of PtDof family genes under low temperature stress,aiming to explore the non CBF dependent cold signaling pathway of coniferous trees in response to low temperature stress,and provide important basis for the analysis of molecular regulatory mechanism of cold resistance in Pinus tabuliformis and stress resistant molecular breeding.[Method]Using transcriptome sequencing to identify differentially expressed genes during the low temperature process in P.tabuliformis,we identified the members of PtDof family genes and conducted bioinformatics analysis,phylogenetic analysis,and expression pattern analysis,screened the key response gene PtDof1 to low temperature stress in PtDof family,and cloned and subcellular localized it.Using agrobacterium mediated inflorescence infiltration method to transform Arabidopsis thaliana,the differences in phenotype and antioxidant enzyme activity between wild-type and overexpressing PtDof1 arabidopsis lines after low temperature treatment were compared and analyzed,and the function of PtDof1 in plant response to low temperature stress was explored.[Result](1)After low temperature treatment,the content of malondialdehyde in needles continued to decrease,while POD activity,SOD activity,and proline content increased.(2)A total of 19 869 differentially expressed genes,including 640 significantly up-regulated genes and 446 significantly down-regulated genes were found in the seedlings of P.tabuliformis at low temperature.(3)A total of 30 Dof genes were identified,and the gene expression patterns in response to low temperature were significantly different,PtDof1 was significantly associated with cold stress.(4)Heterologous transformation showed that the overexpression of PtDof1 improved cold resistance of transgenic Arabidopsis thaliana,and the oxidative stress response was significantly increased.[Conclusion]The PtDof1 gene in P.tabuliformis can respond to low temperature stress and may enhance plant cold tolerance by enhancing antioxidant enzyme activity.The research results have demonstrated the existence of non CBF dependent cold signaling pathways in P.tabuliformis,which not only provides a preliminary theoretical basis for analyzing the function of PtDof1in P.tabuliformis,but also provides important genetic resources for genetic improvement of cold resistant germplasm resources in conifers.关键词
油松/低温胁迫/PtDof1/功能鉴定/非CBF依赖途径/基因表达/抗逆育种Key words
Pinus tabuliformis/low temperature stress/PtDof1/functional qualification/non-CBF-dependent pathway/gene expression/stress-tolerant breeding分类
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马桂影,周成城,曲凯,李伟..油松PtDof基因家族鉴定与PtDof1基因响应低温胁迫的功能分析[J].北京林业大学学报,2025,47(10):84-95,12.基金项目
国家林业和草原局揭榜挂帅项目(202401-07). （202401-07）
