北京林业大学学报2025，Vol.47Issue(10)：108-119,12.DOI:10.12171/j.1000-1522.20240420
脱氢表雄酮对美国白蛾幼虫G6PD活性及几丁质合成的影响
Effects of dehydroepiandrosterone on G6PD activity and chitin synthesis in Hyphantria cunea larvae
摘要
Abstract
[Objective]This study investigated the inhibitory effect of dehydroepiandrosterone(DHEA)on glucose-6-phosphate dehydrogenase(G6PD)activity and the pentose phosphate pathway(PPP)in larvae of Hyphantria cunea,a major agricultural and forestry pest,further examining the role of the PPP in larval growth,development,and chitin biosynthesis.[Method]The HcG6PD gene was cloned from Hyphantria cunea,and the amino acid composition,physicochemical properties and structural characteristics of HcG6PD were analyzed by bioinformatics software.The expression pattern of HcG6PD at different developmental stages was analyzed by RT-qPCR.The G6PD activity,the expression levels of key genes and intermediate product content in PPP in the 5th instar larvae of H.cunea were measured after treating with DHEA.Meanwhile,the inhibitory effect of DHEA on G6PD and PPP was analyzed.To evaluate the effects of G6PD inhibition on chitin synthesis,the relative expression levels of key genes in the chitin synthesis pathway and the chitin content in epidermis of the 5th instar H.cunea larvae were determined.Meanwhile,chitin in the epidermis was stained after treating with DHEA.[Result](1)After treating the 5th instar larvae with DHEA for 72 hours,the activity of G6PD significantly decreased,and the transcriptional levels of the key genes of PPP(HcG6PD,Hc6PGD,Hc6PGL)were only 18%,17%,and 13%of control,respectively.The content of fructose-6-phosphoric acid(F6P)of key genes of PPP was only 0.18 times of control.(2)DHEA treatment led to the obstruction of larval growth and molting.After treating with DHEA for 72 hours,the mass gain of the 5th instar larvae was only 13%of control,and the molting time of larvae was delayed by approximately 4.8 days compared with control.(3)The transcription levels of key genes(HcGNA,HcPAGM,HcUAP,HcCHSA)in chitin synthesis pathway of the 5th instar H.cunea larvae were inhibited by DHEA.This inhibition led to a reduction in epidermal chitin content,which was only 27%of control,as well as a thinning of epidermal chitin structure.[Conclusion]DHEA significantly inhibites the PPP metabolic pathway in H.cunea larvae by suppressing G6PD activity,reducing the transcriptional levels of Hc6PGL and Hc6PGD,and the content of F6P.The inhibition of G6PD activity and PPP induced by DHEA significantly depresses the growth and development,and the epidermal chitin biosynthesis in H.cunealarvae.关键词
葡萄糖-6-磷酸脱氢酶/脱氢表雄酮/美国白蛾/幼虫生长/几丁质生物合成Key words
glucose-6-phosphate dehydrogenase(G6PD)/dehydroepiandrosterone(DHEA)/Hyphantria cunea/larval growth/chitin biosynthesis分类
农业科技引用本文复制引用
木其日,邹传山,王子卓..脱氢表雄酮对美国白蛾幼虫G6PD活性及几丁质合成的影响[J].北京林业大学学报,2025,47(10):108-119,12.基金项目
国家自然科学基金项目(32371880). （32371880）
