传感技术学报2025，Vol.38Issue(9)：1540-1546,7.DOI:10.3969/j.issn.1004-1699.2025.09.002
基于功能性味蕾类器官的仿生传感器用于苦味检测的研究
Functional Taste Bud Organoid-Based Biomimetic Sensor for Bitter Taste Detection
摘要
Abstract
The biological taste system has the unique ability to detect gustatory substances.Biomaterials derived from biological taste sys-tem are considered as ideal sensitive elements for developing taste-based biosensors.Biomimetic taste sensors have inherited the advanta-ges of biochemical sensory systems and have decisive advantages in sensitivity,response time,specificity,and other parameters,which show a broad application prospects in many fields such as food safety,environmental monitoring,and disease detection.In vitro 3D organoids can maximally mimic the state of in vivo organs,which are ideal models for current research.A taste bud organoid-based biosensor for taste sensation is developed.Taste bud organoids prepared from neonatal mice are loaded onto the surface of a 64-channel microelectrode array(MEA)chip to explore the electrophysiological changes during the onset of taste sensation.MEA chip is utilized to record the activity of the spikes from the taste bud organoids under the stimulation of bitter substances,which can help to reveal the role of the taste bud or-ganoids in the perception of taste.The results show that the MEA chip can effectively monitor the electrophysiological signals of taste bud organoids in response to bitter stimuli.This biosensor can not only provide a new approach for the detection of taste substances,but also provide new techniques to study taste perception mechanisms and taste bud function.关键词
味蕾/类器官/生物传感器/味觉感知/MEAKey words
taste bud/organoid/biosensor/taste detection/MEA分类
信息技术与安全科学引用本文复制引用
刘淑阁,陈雅婷,刘鸣皋,王之遥,陈炜,杜立萍,吴春生..基于功能性味蕾类器官的仿生传感器用于苦味检测的研究[J].传感技术学报,2025,38(9):1540-1546,7.基金项目
国家自然科学基金项目(32471433,32271427,32071370) （32471433,32271427,32071370）
国家重点研发计划生物安全关键技术研究重点专项2023年度创新特区项目(2023YFC2606700) （2023YFC2606700）
