吉林医药学院学报2025，Vol.46Issue(5)：328-334,7.
紫苏叶总黄酮与多酚提取工艺优化及其对α-葡萄糖苷酶的抑制活性研究
Optimization of Extraction Process of Total Flavonoids and Polyphenols from Perilla Frutescens Leaves and Their Inhibitory Activity Against α-Glucosidase
摘要
Abstract
Objective The extraction process of total flavonoids and polyphenols from Perilla frutescens leaves was optimized,and their in vitro hypoglycemic activity was evaluated.Methods Using single-factor and orthogonal experiments,the extraction process of total flavo-noids and polyphenols from P.frutescens leaves was optimized.The in vitro hypoglycemic activity of the extracts was evaluated by measur-ing the inhibitory effects on α-glucosidase activity.Results The optimal extraction condition for total flavonoids of P.frutescens leaves was as follows:ethanol concentration of 70%,material-liquid ratio of 1∶55(g∶mL),soaking time of 2 h,ultrasound time of 60 min,ultra-sound temperature of 60℃,and ultrasound power of 400 W.The content of total flavonoids in P.frutescens leaves was(290.30±2.50)mg/g.The optimal condition for total polyphenols of P.frutescens leaves was as follows:ethanol concentration of 40%,liquid-liquid ratio of 1∶35(g∶mL),soaking time of 3 h,ultrasound time of 40 min,and ultrasound power of 350 W.The total polyphenol content was(166.12±1.77)mg/g.The total flavonoids and polyphenol extracts of P.frutescens leaves inhibited α-glucosidase with IC50 values of(32.09±1.13)and(68.17±1.82)mg/L,respectively.Conclusion The optimized extraction process of total flavonoids and polyphenols from P.frutescens leaves was reproducible and reasonably feasible.The total flavonoids and polyphenols extracts of P.frutescens leaves showed good in vitro hypoglycemic activity,which provides reference for their development and utilization.关键词
紫苏叶/总黄酮/总多酚/提取工艺/α-葡萄糖苷酶Key words
Perilla frutescens leaves/total flavonoids/total polyphenols/extraction process/α-glucosidase分类
轻工纺织引用本文复制引用
叶超,吴盈,杨福豪,宋源,王婷婷,吴兆根,刘哲..紫苏叶总黄酮与多酚提取工艺优化及其对α-葡萄糖苷酶的抑制活性研究[J].吉林医药学院学报,2025,46(5):328-334,7.基金项目
吉林省教育厅科学技术研究项目(JJKH20240591KJ,JJKH20240588KJ) （JJKH20240591KJ,JJKH20240588KJ）
吉林医药学院大学生创新创业训练计划项目(2024CXXL071) （2024CXXL071）
