辐射研究与辐射工艺学报2025，Vol.43Issue(5)：54-60,7.DOI:10.11889/j.1000-3436.2025-0041
两种剂量率60Co γ射线诱导淋巴细胞微核的剂量效应
The dose-effects of lymphocyte micronuclei induced by two dose rates of 60Co γ rays
摘要
Abstract
To establish and compare the dose-effect curves of human lymphocyte micronucleus induced by 60Co γ rays at two dose rates.Blood samples in vitro were irradiated with 60Co γ rays at dose rates of 0.318 9 Gy/min and 0.635 0 Gy/min,with absorbed doses of 0 Gy,0.25 Gy,0.50 Gy,0.75 Gy,1 Gy,2 Gy,3 Gy,4 Gy,and 5 Gy.Lymphocyte cultures were performed using the cytokinesis-block micronucleus(CBMN)method.Then the micronucleus(MN)rate and micronucleus cell(MN cell)rate were analyzed and calculated to fit the dose-effect curve.The results showed that under both dose rates,the MN rates increased with increasing absorbed dose,and the fitted dose-effect curves conformed to the quadratic polynomial model.The increase in the MN rate with the absorbed dose at the dose rate of 0.635 0 Gy/min was greater than that at 0.318 9 Gy/min.The results above indicate that the micronuclear dose-effect curves at two dose rates were successfully established,providing technical support and a theoretical basis for more accurate biological dose estimation and study on the biological effects.关键词
60Coγ射线/微核率/剂量率/剂量-效应曲线/胞质分裂阻断微核法Key words
60Co γ-ray/Micronucleus rate/Dose rate/Dose-effect curve/Cytokinesis-block micronucleus method分类
医药卫生引用本文复制引用
吴苹,田钲鉨,谢磊,李启杰..两种剂量率60Co γ射线诱导淋巴细胞微核的剂量效应[J].辐射研究与辐射工艺学报,2025,43(5):54-60,7.基金项目
成都医学院科研基金一般项目(自然科学类)(CYZ19-38) Supported by General Project of Chengdu Medical College Research Fund(Natural Science Category)(CYZ19-38) （自然科学类）
