广东药科大学学报2025，Vol.41Issue(5)：56-63,8.DOI:10.16809/j.cnki.2096-3653.2025050601
余甘子提取物对急性酒精中毒模型大鼠解酒护肝作用研究
Study on the detoxifying and liver-protective effects of Phyllanthus emblica L.extracts on acute alcohol intoxication model rats
摘要
Abstract
Objective To evaluate the detoxifying and liver-protective effects of Phyllanthus emblica L.(PE)on acute alcohol intoxication model rats,and explore its therapeutic mechanism.Methods Forty-two male SD rats were randomly divided into 7 groups,including normal control group,model group,high and low dose groups of PE fruit juice polyphenol extract,high and low dose groups of PE fruit residue polysaccharide extract,and positive control group of Gegen Zhiju compound tablets.In addition to the normal control group,the acute ethanol intoxication model was prepared by gavage of excessive alcohol 0.5 h after gavage of test samples of different concentrations in each experimental group.Serum biomarkers,including aspartate aminotransferase(AST),alanine aminotransferase(ALT),and ethanol levels,were quantified.Hepatic oxidative stress and metabolic parameters,including superoxide dismutase(SOD),malondialdehyde(MDA),glutathione(GSH),alcohol dehydrogenase(ADH),and aldehyde dehydrogenase(ALDH),were also determined.Histopathological assessment of gastric mucosal integrity was performed using hematoxylin and eosin(HE)staining.Results After administering excessive alcohol by gavage,the mental state and activity ability of rats in each treatment group were significantly better than those in the model group.The ethanol content of different groups treated with extracts of PE decreased significantly within 8 hours of drunkenness(P<0.05).The high-dose group of fruit polyphenol extract and fruit residue polysaccharide significantly reduced AST and ALT levels(P<0.01),and increased SOD,GSH,MDA,ADH,and ALDH levels in liver tissue(P<0.05 or P<0.01).Pathological sections of gastric mucosa tissue showed severe bleeding,extensive shedding of epithelial cells,severe tissue edema,and significant infiltration of inflammatory cells in the model group.All dose groups of PE extract improved the pathological changes of rat gastric mucosa tissue to varying degrees.Conclusion Extracts derived from fresh PE fruits can improve the activity of ethanol metabolizing enzymes,enhance antioxidant capacity,accelerate alcohol metabolism,and alleviate liver and stomach tissue damage,showing significant sobering,liver protective,and gastric mucosal protective effects on acute alcohol poisoning.关键词
余甘子/急性酒精中毒/酒精代谢/胃黏膜损伤/保护作用Key words
Phyllanthus emblica L./acute alcohol intoxication/ethanol metabolism/gastric mucosal injury/protective effect分类
医药卫生引用本文复制引用
许彬,吕昊鹏,王淑美,谢媛媛..余甘子提取物对急性酒精中毒模型大鼠解酒护肝作用研究[J].广东药科大学学报,2025,41(5):56-63,8.基金项目
广东省普通高校科技服务乡村振兴重点领域专项(2023ZDZX4024) （2023ZDZX4024）
