广东药科大学学报2025，Vol.41Issue(5)：64-73,10.DOI:10.16809/j.cnki.2096-3653.2025052001
咳嗽宁方治疗小鼠慢性咳嗽的作用及机制探讨
Effects and mechanism of Ke-sou-ning decoction in treating chronic cough in mice
摘要
Abstract
Objective To investigate the therapeutic effect of Ke-sou-ning decoction(KSND)on chronic cough(CC)in mice,and explore its mechanism of action by combining network pharmacology and experimental verification.Methods A CC mouse model was established by inhaling concentrated ammonia water to induce cough.After the model was established,low,medium,and high doses of KSND were administered for intervention treatment.The cough frequency and cough latency of mice were observed and recorded,and the latency extension rate and cough inhibition rate were calculated.HE staining was used to conduct pathological analysis of the trachea and lung tissues.The research method of network pharmacology was employed to screen the active ingredients,key targets,and signaling pathways of KSND for treating CC.The cytometric bead array(CBA)multi-factor flow cytometry kit was used to verify the predicted targets.Results KSND significantly reduced the cough frequency and prolonged the cough latency of CC mice,increased the latency extension rate and cough inhibition rate.It also restructured the damaged tracheal tissue of CC mice,restored the shape of alveoli,and alleviated inflammatory infiltration.The results of network pharmacology research showed that there were 164 potential core active ingredients and 309 potential targets and signaling pathways for KSND in treating CC,including AKT,TNF,STAT3,IL-10,etc.CBA results showed that compared to the model group,the expression level of TNF-α in the bronchoalveolar lavage fluid of mice in the treatment group was significantly reduced,while the expression level of IL-10 was significantly increased.Conclusion KSND can alleviate the symptoms of CC mice and reduce the pathological damage to lung and tracheal tissues,and its mechanism of action may be related to the regulation of inflammatory-immune balance.关键词
慢性咳嗽/网络药理学/咳嗽宁方/小鼠Key words
chronic cough/network pharmacology/Ke-sou-ning decoction/mouse分类
医药卫生引用本文复制引用
舒之挺,韩彬,邓向亮..咳嗽宁方治疗小鼠慢性咳嗽的作用及机制探讨[J].广东药科大学学报,2025,41(5):64-73,10.基金项目
第七批全国老中医药专家学术经验继承工作项目(国中医药人教函[2022]76号) （国中医药人教函[2022]76号）
广东药科大学中医学重点学科建设项目(ZY2021T03) （ZY2021T03）
