广东药科大学学报2025，Vol.41Issue(5)：87-94,8.DOI:10.16809/j.cnki.2096-3653.2024120301
香砂六君子汤通过PERK/eIF2α信号通路调节Cajal间质细胞自噬与凋亡的作用机制
Mechanism of Xiangsha Liujunzi Decoction in regulating autophagy and apoptosis of Cajal interstitial cells via the PERK/eIF2α pathway
摘要
Abstract
Objective To investigate the effect of Xiangsha Liujunzi Decoction on regulation of autophagy and apoptosis of Cajal interstitial cells(ICC)via the PERK/eIF2α pathway,providing experimental evidence for treating functional dyspepsia(FD).Methods FD rat models were induced with iodoacetamide and irregular diet,then divided into normal control(NC),model control(MC),and low-dose(LD),medium-dose(MD),high-dose(HD)Xiangsha Liujunzi Decoction groups.After 14 days of treatment,medicated serum was used to treat ICC cells.Gastric pathology was assessed by HE staining.Cell autophagy was measured by MDC and AO staining.Cell apoptosis was detected by flow cytometry.The expression of PERK,p-eIF2α,eIF2α was detected by western blot.Results MC group showed significant gastric damage,gland disarray,and inflammation,which were notably improved in the HD group.Cell autophagy was elevated in MC and reduced by HD.Cell apoptosis was increased in MC and decreased dose-dependently in LD,MD and HD groups(P<0.05).Western blot revealed PERK and p-eIF2α were decreased and eIF2α was increased compared with MC group.Treatment with Xiangsha Liujunzi Decoction restored PERK and p-eIF2α and reduced eIF2α.Conclusion Xiangsha Liujunzi Decoction inhibits ICC autophagy and apoptosis via the PERK/eIF2α pathway,improves gastrointestinal motility disorders in a dose-dependent manner,supporting FD treatment.关键词
香砂六君子汤/功能性消化不良/Cajal间质细胞/自噬/PERK/eIF2α信号通路Key words
Xiangsha Liujunzi Decoction/functional dyspepsia/Cajal interstitial cells/autophagy/PERK/eIF2α signaling pathway分类
医药卫生引用本文复制引用
李霭燕,招志辉,廖文力,赵荣..香砂六君子汤通过PERK/eIF2α信号通路调节Cajal间质细胞自噬与凋亡的作用机制[J].广东药科大学学报,2025,41(5):87-94,8.基金项目
国家中医药管理局监测统计中心深化医改中医药政策研究(自选课题)(YGZXKT2024317) （自选课题）
广州市番禺区科技计划医疗卫生项目(2024-Z04-058) （2024-Z04-058）
