南方农业学报2025，Vol.56Issue(8)：2390-2408,19.DOI:10.3969/j.issn.2095-1191.2025.08.003
基于广泛靶向代谢组学解析福建省2个产地山金柑果实次生代谢物差异
Analysis of differences in secondary metabolites of Fortunella hindsii Swingle fruits from two production areas in Fujian based on widely targeted metabolomics
摘要
Abstract
[Objective]This study aimed to investigate the metabolome differences and medicinal component charac-teristics of Fortunella hindsii Swingle fruits from two production areas in Fujian Province based on widely targeted me-tabolomics,so as to provide a scientific basis for regional germplasm conservation,quality certification,functional pro-duct development,and high-value utilization of waste.[Method]Fortunella hindsii Swingle fruits from two main produc-tion areas in Fujian Province—Yongchun County in Quanzhou City,and Songxi County in Nanping City—were used as experimental materials.A widely targeted metabolomic analysis of seeds and seed-removed fruits from both production areas was conducted using ultra performance liquid chromatography tandem mass spectrometry(UPLC-MS/MS).Principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA)were employed to screen differential metabolites,and KEGG pathway enrichment analysis was performed to elucidate accumulation pat-terns and functions of the metabolites.[Result]The widely targeted metabolomics analysis identified a total of 1594 se-condary metabolites from seed-removed fruits and seeds of Fortunella hindsii Swingle from the two production areas;the first principal component(PC1)explained 57.60%for the dataset,and the second principal component(PC2)explained 17.86%for the dataset.1119 and 1072 differentially metabolites were detected in different tissue parts of the fruits from the two production areas,among which 884 were tissue-specific differential metabolites.The peak areas of flavonoids and terpenoids in seed-removed fruits from the two production areas were significantly higher than those in seeds(P<0.05,the same below);the peak areas of lignans,coumarins,and quinones were significantly higher in seeds than those in seed-removed fruits.KEGG pathway enrichment analysis indicated that the tissue-specific differential metabolites in tissue parts of fruits from the two production areas were enriched in the anthocyanin biosynthesis pathway.In the 33 core differ-ential metabolites were 10 alkaloids,7 flavonoids,12 lignans and coumarins,2 phenolic acids,and 2 other compounds.Among these,6 core differential metabolites were consistently highly expressed in the seed-removed fruits from the two production areas,including luteolin-6-C-glucoside-7-O-rhamnoside,orientin-2''-O-rhamnoside,luteolin-8-C-glucoside(orientin).Thereby,24 geographical-specific differential metabolites were identified:samples of seed-removed fruits and seeds from Yongchun County had 8 and 11 geographical-specific differential metabolites,respectively;samples of seed-removed fruits and seeds from Songxi County had 3 and 2 geographical-specific differential metabolites,respectively.[Conclusion]Differences in secondary metabolites of Fortunella hindsii Swingle fruits are greater between tissue parts than between geographical origins.Seed-removed fruits are primarily enriched with flavonoids and terpenoids,while seeds are mainly enriched with defensive metabolites such as alkaloids,lignans,and coumarins.A total of 24 geographical-specific differential metabolites between Yongchun County and Songxi County are identified through identification.关键词
山金柑/代谢组学/地理差异/次生代谢物/药用成分Key words
Fortunella hindsii Swingle/metabolomics/geographical difference/secondary metabolites/medicinal components分类
农业科技引用本文复制引用
胡菡青,林雄杰,陈学蓉,赵晓玮,张艳芳,吴梓洋,温寿星,王小安,许家辉..基于广泛靶向代谢组学解析福建省2个产地山金柑果实次生代谢物差异[J].南方农业学报,2025,56(8):2390-2408,19.基金项目
国家自然科学基金面上项目(32472676) （32472676）
福建省科技厅引导性项目(2022N0021) （2022N0021）
福建省农业高质量发展超越创新工程"5511"项目(XTCXGC2021006) （XTCXGC2021006）
福建省科技厅公益类项目(2022R1028001) General Program of National Natural Science Foundation of China(32472676) （2022R1028001）
Guiding Project of Fujian Provincial Department of Science and Technology(2022N0021) （2022N0021）
Fujian Province"5511"Project for High-Quality Agricultural Development Transformative Innovation(XTCXGC2021006) （XTCXGC2021006）
Public Welfare Research Project of Fujian Provincial Department of Science and Technology(2022R1028001) （2022R1028001）
