南方农业学报2025，Vol.56Issue(8)：2439-2456,18.DOI:10.3969/j.issn.2095-1191.2025.08.006
基于质量源于设计理念优化葡萄籽低聚原花青素制备工艺及体外抗氧化活性分析
Optimization of the preparation process for oligomeric proan-thocyanidins from grape seeds based on the quality by design concept and analysis of their in vitro antioxidant activity
摘要
Abstract
[Objective]This study aimed to develop key technologies for grape seed oligomeric proanthocyanidins(GS-OPC)preparation to build a scientific and comprehensive quality control system,and evaluate the GS-OPC antioxi-dant activity,so as to provide a reference for further development and utilization of GS-OPC extracts.[Method]The con-cept of quality by design(QbD)was introduced in risk factor analysis,single-factor experiments,and experimental de-sign to determine the operating space of the preparation process.The GS-OPC antioxidant activity was determined using the methods of 1,1-diphenyl-2-picryl hydrazyl(DPPH),2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS),FRAP(ferric ion reducing antioxidant potential)in experiments of oxidative stress damage induced by hydrogen pero-xide,erastin,and glutamate in PC12 cells.[Result]The GS-OPC preparation process consisted of three steps(ethanol ex-traction,resin enrichment,and ethyl acetate extraction).The operating space for the ethanol-extraction process included 2 extraction times,ethanol volume fraction of 64%-81%,extraction temperature of 30-36℃.The resin enrichment pro-cess included a loading sample ratio of 6∶1(mL/g),ethanol volume fraction of 60%-80%,elution volume of 3.5-4 BV.In the targeted products of GS-OPC,the contents of proanthocyanidins achieved 109.15%and oligomeric proanthocyani-dins 92.05%.Compared with grape seed extracts(GSE)and grape seed proanthocyanidin fractions(GSF),GS-OPC ex-hibited a high antioxidant capacity,with a DPPH free radical scavenging ability of 404.8 mg/g,FRAP total antioxidant capacity of 543.5 mg/g,and an ABTS total antioxidant capacity of 617.6 mg/g.GS-OPC effectively protected cells from oxidative stress damage induced by H2O2,erastin,and Glu by regulating reactive oxygen species(ROS)and lipid hydro-peroxide(LPO);at a dose of 100 μg/mL,the cell survival rate was 89.03%,83.01%,and 92.85%,which represented in-creases of 34.55%,47.70%,and 31.88%(absolute values)compared to the model group,respectively.[Conclusion]Guided by the QbD concept,the development of GS-OPC is scientifically guided,yielding a final product characterized by accessible preparation,controllable quality,and excellent bioactivity.关键词
葡萄籽/低聚原花青素/质量源于设计/制备工艺/操作空间/抗氧化活性Key words
grape seed/oligomeric proanthocyanidins/quality by design/preparation process/operating space/an-tioxidant activity分类
农业科技引用本文复制引用
宋旭超,夏梓铭,李敏,陈立,田瑛,王林..基于质量源于设计理念优化葡萄籽低聚原花青素制备工艺及体外抗氧化活性分析[J].南方农业学报,2025,56(8):2439-2456,18.基金项目
国家自然科学基金项目(82104062) National Natural Science Foundation of China(82104062) （82104062）
