南方农业学报2025，Vol.56Issue(8)：2468-2479,12.DOI:10.3969/j.issn.2095-1191.2025.08.008
铜、锰元素对樟叶越桔组培苗生长及3种酚类物质积累的影响
Effects of copper and manganese elements on the growth and accumulation of three phenolic compounds in tissue-cultured seedlings of Vaccinium dunalianum
摘要
Abstract
[Objective]This study aimed to investigate the effects of two trace elements copper(Cu)and manganese(Mn)on the growth of Vaccinium dunalianum tissue-cultured seedlings and the accumulation of three major phenolic compounds[6'-O-caffeoyl arbutin(CA),β-arbutin(Arb),and chlorogenic acid(CGA)],so as to provide a reference for elucidating the mechanism by which trace elements regulate the growth of tissue-cultured seedlings and targeted accumula-tion of phenolic compounds in Vaccinium dunalianum.[Method]Tissue-cultured seedlings of Vaccinium dunalianum were used as experimental materials.Based on the concentrations of Cu and Mn trace elements in the 1/4 MS medium,five con-centration treatments(0,0.5,1.0,1.5,and 2.0×)for each trace element were established.After 70 d of cultivation,growth indicators including plant water content,plant height,root length,rooting rate,and number of roots per plant,as well as the contents of Arb,CGA,and CA were measured.The relative expression of genes associated with the biosynthe-sis of these three phenolic compounds were determined by real-time fluorescence quantitative PCR.[Result]With increas-ing Cu concentration in the media,the rooting rate and root length of Vaccinium dunalianum tissue-cultured seedlings ex-hibited a gradual decreasing trend.The rooting rate,root length,and number of roots per plant in the Cu(2.0)treatment were significantly lower than those in the Cu(1.0)treatment(P<0.05,the same below).Except for the Cu(2.0)treat-ment,the plant height was significantly lower in all other treatments compared with Cu(1.0)treatment,and the Cu(1.0)treatment exhibited the highest water content at 89.39%.The Mn(2.0)treatment showed significantly higher rooting rate,root length,and number of roots per plant than the other three treatments did,except for the Mn(1.5)treatment.Under both elements treatments,Vaccinium dunalianum tissue-cultured seedlings exhibited optimal growth performance in the 1.0×groups,with the three phenolic compounds ranked as CA>Arb>CGA.Under the Cu(2.0)treatment,the highest Arb and CA contents in Vaccinium dunalianum were observed,at 2.71 and 30.81 mg/g,respectively.The Mn(1.0)treatment yielded the highest CGA content,which significantly exceeded that of the other four treatments.Under the Cu(2.0)treat-ment and compared with the Cu(1.0)treatment,the relative expressions of VdAS-1 and VdAS-2 genes were significantly decreased and the relative expression of VdAS-4 gene was significantly increased;the relative expression of VdAT-3 gene was significantly decreased under Cu(0),Cu(1.5)and Cu(2.0)treatments,while only the relative expression of VdC-CoAOMT-2 gene was significantly increased under Cu(2.0)treatment.Under Mn(2.0)treatment and compared with the Mn(1.0)treatment,the relative expressions of VdAS-1 and VdAS-2 genes were significantly decreased,the relative ex-pression of VdAS-4 gene was significantly increased,and the relative expression of VdAT-1 gene was significantly de-creased.However,under the Mn(2.0)treatment,the relative expression of VdAT-3 gene was significantly increased,the relative expressions of the VdC3H-2,VdC3H-3,VdCCoAOMT-1 and VdHCT genes were significantly decreased,while those of VdCCoAOMT-2 and VdCCoAOMT-3 were significantly increased.[Conclusion]Excessive or deficient Cu inhibits the growth of Vaccinium dunalianum tissue-cultured seedlings.A 2.0×Mn concentration promotes roots development but suppresses the growth of above-ground parts of Vaccinium dunalianum tissue-cultured seedlings.The two phenolic com-pounds CA and Arb play crucial roles in Vaccinium dunalianum defence against Cu and Mn stresses.关键词
樟叶越桔/微量元素/生长发育/酚类物质/基因表达Key words
Vaccinium dunalianum/trace element/growth and development/phenolic compounds/gene expression分类
农业科技引用本文复制引用
杨丽红,李国泽,王凤,常玮,赵展平,丁勇..铜、锰元素对樟叶越桔组培苗生长及3种酚类物质积累的影响[J].南方农业学报,2025,56(8):2468-2479,12.基金项目
国家自然科学基金项目(31960073) （31960073）
云南省中青年学术与技术带头人后备人才项目(202405AC350032) （202405AC350032）
云南省大学生创新训练项目(S202210677033) （S202210677033）
生物学云南省学科建设经费(32004217) National Natural Science Foundation of China(31960073) （32004217）
Yunnan Province Reserve Talents Project for Middle-aged and Young Academic and Technical Leaders(202405AC350032) （202405AC350032）
Yunnan Province College Stu-dents'Innovation Training Project(S202210677033) （S202210677033）
Provincial Funding for Biology Discipline Construction(32004217) （32004217）
