南方农业学报2025，Vol.56Issue(8)：2510-2518,9.DOI:10.3969/j.issn.2095-1191.2025.08.012
水杨酸生物合成途径对薄壳山核桃果实膨大期幼果脱落的调控机制
Regulation mechanisms of salicylic acid biosynthesis pathway on nutlet abscission of Carya illinoinensis(Wangenheim)K.Koch during nut sizing stage
摘要
Abstract
[Object]This study aimed to investigate functions of salicylic acid(SA)and its biosynthesis pathway on regulating nutlet abscission at the nut sizing stage of Carya illinoinensis(Wangenheim)K.Koch,so as to elucidate the in-ternal causes of nutlet abscission,and provide a new perspectives for the high and stable yield of Carya illinoinensis(Wan-genheim)K.Koch.[Method]The nuts of Pawnee variety of Carya illinoinensis(Wangenheim)K.Koch were taken as materials,the SA contents in abscised and normally developed nutlets were measured at 53 days after pollination to ana-lyze effects of SA treatment on the nutlet abscising and development during the nut sizing stage of Carya illinoinensis(Wangenheim)K.Koch.Based on transcriptome data,the expressions of genes encoded by two key enzymes—phenylala-nine ammonia-lyase(PAL)and isochorismate synthase(ICS)—were analyzed between the abscising and normally deve-loped nutlets.The gene structure,chromosome location and phylogenetic tree of PAL gene family members in Carya illi-noinensis(Wangenheim)K.Koch were analyzed.[Result]The SA content in abscising nutlets was significantly higher than that in normally developed nutlets at 53 days after pollination(P<0.05,the same below).The nut abscising rate of SA treatment group was highly significantly higher than that of control(water group)after SA treatment for 10 d(P<0.01,the same below).The single nut mass in SA treatment group was significantly or highly significantly lower than that of the control group at after 7,10 and 13 d of SA treatment.Under normal growth conditions,the single nut mass of abscising nutlets was significantly lower than that of the normally developed nutlets;all the developmentally retarded or arrested nuts will abscise within the following 7 d.The FPKM of genes CiPAL5 and CiPAL6 in abscising nutlets were significantly up-regulated comparing with normally developed nutlets.There were six PAL gene family members(CiPAL1-CiPAL6)in the Carya illinoinensis(Wangenheim)K.Koch genomes,and the three up-regulated CPAL4-6 genes with similar struc-tures were closely arranged in tandem on Chromosome No.4.Phylogenetic tree results showed that proteins CiPAL1-CiPAL3 shared a close relationship with PAL proteins of dicotyledons such as Arabidopsis thaliana,tobacco,grape,and poplar.Proteins encoded by CiPAL4-CiPAL6 tandem gene clusters located in Chromosome No.4 of Carya illinoinensis(Wangenheim)K.Koch were cluster with proteins encoded by VviPAL3-VviPAL6 tandem gene clusters located in Chro-mosome No.11 of grape.[Conclusion]SA treatment inhibits the nut development of Carya illinoinensis(Wangenheim)K.Koch and promotes nutlet abscising,suggesting that SA plays an important role in the progress of nutlet abscising dur-ing the nut sizing stage.PAL pathway is the main SA biosynthesis pathway in the process of nutlet abscising during the nut sizing stage and regulates the nutlet abscising of Carya illinoinensis(Wangenheim)K.Koch.关键词
薄壳山核桃/幼果脱落/水杨酸/苯丙氨酸解氨酶(PAL)Key words
Carya illinoinensis(Wangenheim)K.Koch/nutlet abscissing/salicylic acid/phenylalanine ammonia-lyase(PAL)分类
农业科技引用本文复制引用
张计育,王雪凡,贾展慧,王涛,陈智坤,王刚,宣继萍..水杨酸生物合成途径对薄壳山核桃果实膨大期幼果脱落的调控机制[J].南方农业学报,2025,56(8):2510-2518,9.基金项目
江苏省农业科技自主创新资金项目[CX(24)3047] （24）
江苏省自然科学基金面上项目(BK20231394) （BK20231394）
陕西省林业科技创新重点专项(SXLK2022-02-5) Jiangsu Fund Project for Agricultural Science and Technology Independent Innovation[CX(24)3047] （SXLK2022-02-5）
General Project of Natural Science Foundation of Jiangsu(BK20231394) （BK20231394）
Major Project of Forestry Science and Technology Innovation of Shaanxi Province(SXLK2022-02-5) （SXLK2022-02-5）
